大鼠嗅结节和Calleja岛中的神经发生

Neurogenesis in the olfactory tubercle and islands of Calleja in the rat.

作者信息

Bayer S A

机构信息

Department of Biology, Indiana-Purdue University, 1125 East 38th Street, Indianapolis, IN 46223, U.S.A.

出版信息

Int J Dev Neurosci. 1985;3(2):135-47. doi: 10.1016/0736-5748(85)90004-8.

DOI:10.1016/0736-5748(85)90004-8

PMID:24874595

Abstract

Neurogenesis in the rat olfactory tubercle and islands of Calleja was examined with [(3)H]thymidine autoradiography. Animals in the prenatal groups were the offspring of pregnant females given an injection of [(3)H]thymidine on two consecutive gestational days. Ten groups of embryos (E) were exposed to [(3)H]thymidine on E12-E13, E13-E14 4 E21-E22, respectively. Three groups of postnatal animals (P) were given four consecutive injections of [(3)H]thymidine on P0-P3, P2-P5, and P4-P7, respectively. On P60, the percentage of labeled cells and the proportion of cells originating during either 24 or 48 h periods were quantified at several anatomical levels. Three populations of neurons were studied:

摘要

采用[³H]胸腺嘧啶核苷放射自显影术对大鼠嗅结节和Calleja岛中的神经发生进行了研究。产前组动物是在连续两个妊娠日接受[³H]胸腺嘧啶核苷注射的怀孕雌性动物的后代。十组胚胎（E）分别在胚胎第12 - 13天、第13 - 14天和第21 - 22天暴露于[³H]胸腺嘧啶核苷。三组产后动物（P）分别在出生后第0 - 3天、第2 - 5天和第4 - 7天接受连续四次[³H]胸腺嘧啶核苷注射。在出生后第60天，在几个解剖层面上对标记细胞的百分比以及在24小时或48小时时间段内产生的细胞比例进行了量化。研究了三类神经元：