Adamson Teagan L, Cook Curtiss B, LaBelle Jeffrey T
Harrington Biomedical Engineering Program in the School of Biological and Health Systems Engineering, Arizona State University, Tempe, AZ, USA.
Division of Endocrinology and Division of Preventive, Occupational, and Aerospace Medicine, Mayo Clinic Arizona, Scottsdale, AZ, USA.
J Diabetes Sci Technol. 2014 Mar;8(2):350-355. doi: 10.1177/1932296814523874. Epub 2014 Feb 21.
Multiple markers are used to assess glycemic control in patients with diabetes mellitus (DM). New technology that permits simultaneous detection of multiple biomarkers combined with those used at the point of care indicative of glycemic control, including glycemic variability determined from 1,5-anhydroglucitol measurement, could provide better management and further insight into the disease. This platform was based on previous research involving glucose detection and uses electrochemical impedance spectroscopy to detect a range of 1,5-anhydroglucitol concentrations at an optimal binding frequency. The enzyme pyranose oxidase was fixed to gold electrodes while a sine wave of sweeping frequencies was induced in purified solutions and in variable presence of whole blood. The optimal binding frequency for the detection of 1,5-anhydroglucitol was found to be 3.71 kHz. The impedance response compared to the concentration of target present was found to have a logarithmic slope of 7.04 with an R-squared value of 0.96. This response includes 2 experimental sets, a single test of a low concentration range and a high concentration range with 5 replicates. The relative standard deviation of the high range varied from 28% to 27% from lowest to highest concentrations. Best detection in complex solutions was found in lower blood concentrations of 0.5% and 1%, but maintained relatively high accuracy in concentrations 5% and 10%. The sensor platform was successfully evaluated at a high dynamic range of 1,5-AG in purified solutions. In the presence of whole blood, lowest percentages yielded the best results indicating that filtering interferents may be necessary in final device architecture.
多种标志物用于评估糖尿病（DM）患者的血糖控制情况。新技术能够同时检测多种生物标志物，并结合即时检测中用于指示血糖控制的标志物，包括通过1,5-脱水葡萄糖醇测量确定的血糖变异性，这可能会为该疾病提供更好的管理并带来更深入的了解。该平台基于先前涉及葡萄糖检测的研究，并利用电化学阻抗谱在最佳结合频率下检测一系列1,5-脱水葡萄糖醇浓度。将吡喃糖氧化酶固定在金电极上，同时在纯化溶液和不同全血存在情况下诱导扫频正弦波。发现检测1,5-脱水葡萄糖醇的最佳结合频率为3.71 kHz。与存在的目标物浓度相比，阻抗响应的对数斜率为7.04，决定系数R²值为0.96。该响应包括2个实验组，一个是低浓度范围的单次测试，另一个是高浓度范围的5次重复测试。高浓度范围的相对标准偏差从最低浓度到最高浓度在28%至27%之间变化。在0.5%和1%的较低血液浓度下，在复杂溶液中检测效果最佳，但在5%和10%的浓度下仍保持相对较高的准确性。该传感器平台在纯化溶液中1,5-AG的高动态范围内成功进行了评估。在全血存在的情况下，最低百分比产生了最佳结果，这表明在最终设备架构中可能需要过滤干扰物。
