Harrington Biomedical Engineering Program in the School of Biological and Health Systems Engineering, Arizona State University, The Biodesign Institute, Tempe, 85287-9709, USA.
Analyst. 2012 Sep 21;137(18):4179-87. doi: 10.1039/c2an35645g. Epub 2012 Jul 30.
Self-monitoring of blood glucose is the standard of care in management of hyperglycemia among patients with diabetes mellitus. To increase the sensitivity and specificity of current devices, a novel method of detecting glucose using electrochemical impedance spectroscopy (EIS) technology is explored. The enzyme glucose oxidase (GOx) was fixed to gold electrodes and a sine wave of sweeping frequencies was induced using a wide range of concentrations of glucose. Each frequency in the impedance sweep was analyzed for the highest response and R-squared value. The frequency with both factors optimized is specific for the glucose-GOx binding interaction and was determined to be 1.17 kHz in purified solutions in both higher and lower ranges of glucose. The correlation between the impedance response and concentration at the low range of detection (0-100 mg dL(-1) of glucose) was determined to be 3.53 ohm/ln (mg dL(-1)) with an R-squared value of 0.90 with a 39 mg dL(-1) lower limit of detection. The same frequency of 1.17 kHz was verified in whole blood under the same glucose range. The above data confirm that EIS offers a new method of glucose detection as an alternative to current technology in use by patients. Additionally, the unique frequency response of individual markers allows for modulation of signals so that several other markers important in the management of diabetes could be measured with a single sensor.
自我血糖监测是糖尿病患者高血糖管理的标准护理方法。为了提高现有设备的灵敏度和特异性，探索了一种使用电化学阻抗谱（EIS）技术检测葡萄糖的新方法。将葡萄糖氧化酶（GOx）固定在金电极上，并使用广泛浓度的葡萄糖诱导正弦波扫频。在阻抗扫频中分析每个频率的最高响应和 R 平方值。优化这两个因素的频率是特定于葡萄糖-GOx 结合相互作用的，并确定在纯溶液中为 1.17 kHz，在葡萄糖的高低范围内均如此。在低检测范围（0-100mg dL(-1)葡萄糖）下，阻抗响应与浓度之间的相关性确定为 3.53 ohm/ln（mg dL(-1)），R 平方值为 0.90，检测下限为 39mg dL(-1)。相同的 1.17 kHz 频率在相同的葡萄糖范围内在全血中得到了验证。上述数据证实 EIS 提供了一种替代当前患者使用的技术的葡萄糖检测新方法。此外，单个标志物的独特频率响应允许对信号进行调制，从而可以使用单个传感器测量糖尿病管理中其他几个重要的标志物。
