Jung Kyung-Hwa, Choi Hei-Lim, Park Soojin, Lee Geunhyeog, Kim Miran, Min Joon-Ki, Min Byung-Il, Bae Hyunsu
Department of Physiology, College of Korean Medicine, Kyung Hee University, #1 Hoekidong, Dongdaemoon-ku, Seoul 130-701, Republic of Korea.
Department of East-West Medicine, Kyung Hee University, #1 Hoekidong, Dongdaemoon-ku, Seoul 130-701, Republic of Korea.
J Ethnopharmacol. 2014 Aug 8;155(1):113-22. doi: 10.1016/j.jep.2014.04.029. Epub 2014 May 28.
PM014 is a modified form of the Chung-Sang-Bo-Ha-Tang (CSBHT) herbal formula that has been used to treat chronic pulmonary diseases in Korea for centuries. Previously, we developed a formulation of PM014 based on a series of in vitro and in vivo screening efforts that comprises seven herbal extracts. The PM014 formula includes the root of Rehmannia glutinosa, the cortex of Paeonia suffruticosa, the fruit of Schizandra chinensis, the root of Asparagus cochinchinensis, seeds of Prunus armeniaca, the root of Scutellaria baicalensis and the root of Stemona sessilifolia. Asthma is a chronic inflammatory disease of the lungs that is characterized by wheezing, bronchial contraction, and chest tightness. In addition, the airway becomes hypersensitive and narrows through an inflammatory reaction mediated by Th2 cells. The present study was conducted to evaluate the ability of PM014 to prevent allergic airway inflammation and to attenuate airway responses in a cockroach allergen-induced mouse model.
Mice sensitized to and challenged with cockroach allergen were treated with oral administration of PM014. Airway resistance was determined by whole body plethysmography. In addition, Th2 cytokines and immune cell profiles of bronchoalveolar lavage (BAL) fluid and inflammatory mediators in serum were analyzed by ELISA. A series of histological examinations were also conducted to demonstrate the effects of PM014 on airway remodeling, goblet cell hyperplasia and inflammatory responses in the lung.
PM014 significantly inhibited the number of total cells, eosinophils, neutrophils, macrophages and lymphocytes in the BAL fluid of mice that were challenged with cockroach allergen. In addition, PM014 reduced the levels of Th2 cytokines (IL-4, IL-5 and IL-13) in the BAL fluid and inflammatory mediators such as IgE in the serum, as measured by enzyme-linked immunosorbent assay (ELISA). Histopathological analysis also showed that PM014 substantially inhibited eosinophil infiltration into the airway, goblet cell hyperplasia and smooth muscle hypertrophy.
In this study, our results indicate that PM014 has significant effects on allergic airway inflammation upon exposure to cockroach allergen in a mouse model. According to these outcomes, PM014 may have therapeutic potential as a treatment for allergic asthma.
PM014是对中桑补肺汤（CSBHT）草药配方进行改良后的制剂，该配方在韩国用于治疗慢性肺部疾病已有数百年历史。此前，我们基于一系列体外和体内筛选工作，开发了一种包含七种草药提取物的PM014制剂。PM014配方包括熟地黄根、牡丹皮、五味子果实、天冬根、杏仁种子、黄芩根和百部根。哮喘是一种肺部慢性炎症性疾病，其特征为喘息、支气管收缩和胸闷。此外，气道通过Th2细胞介导的炎症反应变得过敏并变窄。本研究旨在评估PM014在蟑螂过敏原诱导的小鼠模型中预防过敏性气道炎症和减轻气道反应的能力。
对蟑螂过敏原致敏并激发的小鼠口服给予PM014进行治疗。通过全身体积描记法测定气道阻力。此外，通过酶联免疫吸附测定（ELISA）分析支气管肺泡灌洗（BAL）液中的Th2细胞因子和免疫细胞谱以及血清中的炎症介质。还进行了一系列组织学检查，以证明PM014对气道重塑、杯状细胞增生和肺部炎症反应的影响。
PM014显著抑制了用蟑螂过敏原激发的小鼠BAL液中总细胞、嗜酸性粒细胞、中性粒细胞、巨噬细胞和淋巴细胞的数量。此外，通过酶联免疫吸附测定（ELISA）测量，PM014降低了BAL液中Th2细胞因子（IL-4、IL-5和IL-13）的水平以及血清中炎症介质如IgE的水平。组织病理学分析还表明，PM014显著抑制了嗜酸性粒细胞向气道的浸润、杯状细胞增生和平滑肌肥大。
在本研究中，我们的结果表明，在小鼠模型中，PM014在暴露于蟑螂过敏原时对过敏性气道炎症具有显著作用。根据这些结果，PM014作为过敏性哮喘的治疗方法可能具有治疗潜力。
