Participation of the membrane effect to the cytotoxicity of tensio-active agents comparison with ocular irritation in vivo.

A study for the comparison of the action of tensio-active agents have been made with 3 models: The rabbit erythrocyte to evaluate the immediate membrane effect. SIRC fibroblasts (rabbit cornea) to evaluate the toxicity after 3 days. The rabbit eye (Draize). The cellular model have shown that there is a difference between non irritants tensio-active agents, and irritants-very irritants in vivo, except the BRIJ group. To evaluate the part of membrane effect in the interaction tensio-active-cell, it has been realized a test of haemolysis on the rabbit erythrocyte on 10 tensio-active agents. It shows that the cellular model can also determine a different toxicity than the one provoked by an aggression on the plasmic membrane.