卡利齐里提取物上调小鼠B16黑色素瘤细胞中酪氨酸酶、TRP-1、TRP-2和MITF的表达。
Kaliziri extract upregulates tyrosinase, TRP-1, TRP-2 and MITF expression in murine B16 melanoma cells.
作者信息
Tuerxuntayi Adila, Liu Yong-qiang, Tulake Ablajan, Kabas Maidina, Eblimit Aiden, Aisa Haji Akber
机构信息
The Key Laboratory of Plant Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China.
出版信息
BMC Complement Altern Med. 2014 May 22;14:166. doi: 10.1186/1472-6882-14-166.
BACKGROUND
Kaliziri extract (KZE) is a traditional Uyghur medicine (TUM), used by traditional hospitals in China as an injection for treatment of vitiligo for more than 30 years. Clinical application has shown that this medicine has obvious therapeutic effects. However, its phytochemical analysis and mechanism have not been examined.
METHODS
KZE was extracted from seeds of Kaliziri [Vernonia anthelmintica (L.) Willd.] in ethanol-water (80:20, v/v), its components were identified by LC-MS/MS, and the signaling pathway of melanin synthesis in KZE treated murine B16 melanoma cells was examined by western blotting.
RESULTS
Liquid chromatography-mass spectrometry analysis confirmed that the main components of KZE are flavonoids. KZE increased the tyrosinase activity and melanin content in a dose-dependent manner at concentrations of 5-40 μg/ml, and treatment with 20 μg/ml of KZE enhanced the expression of tyrosinase in B16 cells in a time-dependent manner.
CONCLUSIONS
KZE induced melanogenesis by increasing the expression of TYR, TRP-1, TRP-2 and MITF in B16 cells.
背景
卡里齐里提取物（KZE）是一种传统维吾尔药（TUM），在中国传统医院用作注射剂治疗白癜风已有30多年。临床应用表明该药具有明显的治疗效果。然而，其植物化学分析及其作用机制尚未得到研究。
方法
用乙醇-水（80:20，v/v）从卡里齐里[驱虫斑鸠菊（Vernonia anthelmintica (L.) Willd.）]种子中提取KZE，通过液相色谱-串联质谱法（LC-MS/MS）鉴定其成分，并用蛋白质印迹法检测KZE处理的小鼠B16黑色素瘤细胞中黑色素合成的信号通路。
结果
液相色谱-质谱分析证实KZE的主要成分是黄酮类化合物。在5-40μg/ml浓度下，KZE以剂量依赖性方式增加酪氨酸酶活性和黑色素含量，用20μg/ml KZE处理以时间依赖性方式增强B16细胞中酪氨酸酶的表达。
结论
KZE通过增加B16细胞中TYR、TRP-1、TRP-2和MITF的表达诱导黑色素生成。
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