Pedrazza Leonardo, Lunardelli Adroaldo, Luft Carolina, Cruz Carolina Uribe, de Mesquita Fernanda Cristina, Bitencourt Shanna, Nunes Fernanda Bordignon, de Oliveira Jarbas Rodrigues
Laboratório de Pesquisa em Biofísica Celular e Inflamação, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Avenida Ipiranga 6681, prédio 12, bloco C, sala 221, Porto Alegre, Rio Grande do Sul, CEP 90619-900, Brazil.
Inflamm Res. 2014 Sep;63(9):719-28. doi: 10.1007/s00011-014-0745-1. Epub 2014 Jun 3.
Mesenchymal stem cells (MSCs) are potent modulators of immune responses. Sepsis is the association of a systemic inflammatory response with an infection. The aim of this study was to test the ability of MSCs derived from adipose tissue, which have immunomodulatory effects, and to inhibit the septic process in an experimental model of mice.
Three experimental groups (male C57BL/6 mice) were formed for the test: control group, untreated septic group and septic group treated with MSCs (1 × 10(6) cells/animal).
In the control group, there were no deaths; in the untreated septic group, the mortality rate was 100 % within 26 h; in the septic group treated with MSCs, the mortality rate reached 40 % within 26 h. The group treated with MSCs was able to reduce the markers of tissue damage in the liver and pancreas. The treated group had a reduction of inflammatory markers. Furthermore, the MSCs-treated group was able to inhibit the increase of apoptosis in splenocytes observed in the untreated septic group.
Our data showed that MSCs ameliorated the immune response with decrease of inflammatory cytokines and increase anti-inflammatory IL-10; moreover, inhibited splenocytes apoptosis and, consequently, inhibited tissue damage during sepsis.
间充质干细胞（MSCs）是免疫反应的有效调节因子。脓毒症是全身炎症反应与感染的关联。本研究的目的是测试源自脂肪组织的具有免疫调节作用的MSCs在小鼠实验模型中抑制脓毒症进程的能力。
为该测试组建了三个实验组（雄性C57BL/6小鼠）：对照组、未治疗的脓毒症组和用MSCs治疗的脓毒症组（1×10⁶个细胞/只动物）。
对照组无死亡；未治疗的脓毒症组在26小时内死亡率为100%；用MSCs治疗的脓毒症组在26小时内死亡率达40%。用MSCs治疗的组能够降低肝脏和胰腺中的组织损伤标志物。治疗组的炎症标志物减少。此外，用MSCs治疗的组能够抑制在未治疗的脓毒症组中观察到的脾细胞凋亡增加。
我们的数据表明，MSCs改善了免疫反应，炎症细胞因子减少，抗炎性白细胞介素-10增加；此外，抑制了脾细胞凋亡，从而在脓毒症期间抑制了组织损伤。
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