Hersini Kamelia J, Melgaard Line, Gazerani Parisa, Petersen Lars J
Department of Health Science and Technology,, Aalborg University, DK-9220 Aalborg, Denmark.
Acta Derm Venereol. 2014 Sep;94(5):501-11. doi: 10.2340/00015555-1878.
Skin microdialysis is an established method for in vivo sample collection from the extracellular fluid space. This method has been extensively used in studies of inflammatory reactions in the skin of animals and humans. Skin microdialysis consists of the implantation of semi-permeable probes into the upper dermis, perfusion with a physiological buffer, and the recovery of the substances that diffused from the skin into the perfusion fluid. Microdialysis allows the simultaneous assessment of the temporal variations of inflammatory mediator release in the skin as well as the monitoring of vascular and sensory functions. By the aid of this technique, potential associations can be found between functional changes and a variety of substances and mediators released at the site of interest. This allows further insights into the possible mechanisms underlying physiological and pathophysiological events in the skin, including cutaneous inflammation. This review provides a comprehensive but not exhaustive review of the use of microdialysis in studies of experimental and clinical inflammatory reactions in the skin in animals and humans.
皮肤微透析是一种从细胞外液空间进行体内样本采集的成熟方法。该方法已广泛应用于动物和人类皮肤炎症反应的研究。皮肤微透析包括将半透性探针植入真皮上层，用生理缓冲液灌注，并回收从皮肤扩散到灌注液中的物质。微透析能够同时评估皮肤中炎症介质释放的时间变化以及监测血管和感觉功能。借助该技术，可以发现功能变化与在感兴趣部位释放的各种物质和介质之间的潜在关联。这有助于进一步深入了解皮肤生理和病理生理事件（包括皮肤炎症）背后的可能机制。本综述全面但非详尽地回顾了微透析在动物和人类皮肤实验性和临床炎症反应研究中的应用。
