Iwasaki Motoki, Mukai Tomomi, Takachi Ribeka, Ishihara Junko, Totsuka Yukari, Tsugane Shoichiro
Division of Epidemiology, Research Center for Cancer Prevention and Screening, National Cancer Center, 5-1-1 Tsukiji, Chuo-ku, Tokyo, 104-0045, Japan,
Cancer Causes Control. 2014 Aug;25(8):1015-28. doi: 10.1007/s10552-014-0401-7. Epub 2014 Jun 3.
Clarification of the putative etiologic role of heterocyclic aromatic amines (HAAs) in the development of cancer requires a validated assessment tool for dietary HAAs. This study primarily aimed to evaluate the validity of a food frequency questionnaire (FFQ) in estimating HAA intake, using 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) level in human hair as the reference method.
We first updated analytical methods of PhIP using liquid chromatography-electrospray ionization/tandem mass spectrometry (LC-ESI/MS/MS) and measured 44 fur samples from nine rats from a feeding study as part-verification of the quantitative performance of LC-ESI/MS/MS. We next measured PhIP level in human hair samples from a validation study of the FFQ (n = 65). HAA intake from the FFQ was estimated using information on intake from six fish items and seven meat items and data on HAA content in each food item. Correlation coefficients between PhIP level in human hair and HAA intake from the FFQ were calculated.
The animal feeding study of PhIP found a significant dose-response relationship between dosage and PhIP in rat fur. Mean level was 53.8 pg/g hair among subjects with values over the limit of detection (LOD) (n = 57). We found significant positive correlation coefficients between PhIP in human hair and HAA intake from the FFQ, with Spearman rank correlation coefficients of 0.35 for all subjects, 0.21 for subjects with over LOD values, and 0.34 for subjects with over limit of quantification.
Findings from the validation study suggest that the FFQ is reasonably valid for the assessment of HAA intake.
阐明杂环芳香胺（HAAs）在癌症发生中假定的病因学作用需要一种经过验证的膳食HAAs评估工具。本研究主要旨在以人发中2-氨基-1-甲基-6-苯基咪唑[4,5-b]吡啶（PhIP）水平作为参考方法，评估食物频率问卷（FFQ）在估计HAAs摄入量方面的有效性。
我们首先使用液相色谱-电喷雾电离/串联质谱（LC-ESI/MS/MS）更新了PhIP的分析方法，并测量了来自一项喂养研究的9只大鼠的44份毛发样本，作为对LC-ESI/MS/MS定量性能的部分验证。接下来，我们测量了来自FFQ验证研究的人发样本（n = 65）中的PhIP水平。使用来自六种鱼类和七种肉类的摄入量信息以及每种食物中HAA含量的数据，估计FFQ中的HAA摄入量。计算人发中PhIP水平与FFQ中HAA摄入量之间的相关系数。
PhIP的动物喂养研究发现大鼠毛发中剂量与PhIP之间存在显著的剂量反应关系。在检测值超过检测限（LOD）的受试者中（n = 57），平均水平为53.8 pg/g毛发。我们发现人发中的PhIP与FFQ中的HAA摄入量之间存在显著的正相关系数，所有受试者的Spearman等级相关系数为0.35，检测值超过LOD的受试者为0.21，定量限以上的受试者为0.34。
验证研究的结果表明，FFQ在评估HAA摄入量方面具有合理的有效性。
