Kobayashi Minatsu, Hanaoka Tomoyuki, Tsugane Shoichiro
Epidemiology and Prevention Division, Research Center for Cancer Prevention and Screening, National Cancer Center, Tokyo 104-0045, Japan.
Mutat Res. 2007 Jun 15;630(1-2):14-9. doi: 10.1016/j.mrgentox.2007.02.003. Epub 2007 Feb 21.
Several case-control studies have reported possible associations between heterocyclic amine (HCA) intake and the risk of cancer. The validity of questionnaires used to assess HCA intake has hardly been examined, however; in particular, no biomarker able to serve as an independent measure of habitual HCA intake has been established. In this study, we examined the validity of HCA intake estimated from a food frequency questionnaire (FFQ) using 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) level in hair as a reference method. Study subjects were 20 volunteers (7 men and 13 women) aged 25-57 years residing in Tokyo or neighboring cities in Japan. The subjects completed the FFQ, and gave 3-5g of hair twice at an interval of 1-3 months for use in establishing validity. Results showed that intakes of PhIP, MeIQ, Trp-P-1, and total HCA by the FFQ were significantly correlated with PhIP levels in hair when adjustment was made for melanin content (r=0.47, r=0.50, r=0.55, and r=0.51, respectively). The present study indicates that HCA intake estimated from this FFQ provides a reasonable ranking of individuals to allow the analysis of associations between HCA intake and risk of cancer in large-scale epidemiological studies.
多项病例对照研究报告了杂环胺（HCA）摄入量与癌症风险之间可能存在的关联。然而，用于评估HCA摄入量的问卷的有效性几乎未得到检验；特别是，尚未建立能够作为习惯性HCA摄入量独立衡量指标的生物标志物。在本研究中，我们以头发中2-氨基-1-甲基-6-苯基咪唑并[4,5-b]吡啶（PhIP）水平作为参考方法，检验了通过食物频率问卷（FFQ）估算的HCA摄入量的有效性。研究对象为居住在日本东京或周边城市的20名志愿者（7名男性和13名女性），年龄在25至57岁之间。受试者完成了FFQ，并每隔1至3个月提供两次3 - 5克头发用于有效性验证。结果表明，在对黑色素含量进行校正后，FFQ得出的PhIP、MeIQ、Trp-P-1和总HCA摄入量与头发中的PhIP水平显著相关（分别为r = 0.47、r = 0.50、r = 0.55和r = 0.51）。本研究表明，通过该FFQ估算的HCA摄入量为个体提供了合理的排序，以便在大规模流行病学研究中分析HCA摄入量与癌症风险之间的关联。
