Hachisuka S, Kamei N, Ujigo S, Miyaki S, Yasunaga Y, Ochi M
Department of Orthopaedic Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.
1] Department of Orthopaedic Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan [2] Translational Research Medical Center, Hiroshima University Hospital, Hiroshima, Japan.
Spinal Cord. 2014 Aug;52(8):596-600. doi: 10.1038/sc.2014.86. Epub 2014 Jun 3.
An in vivo study in mouse models of spinal cord contusion.
To develop a novel indicator to anticipate the severity of spinal cord injury (SCI) during the acute phase and for the assessment of the efficacy of novel therapies. MicroRNAs (miRNAs) circulating in the peripheral blood are reported to modulate signaling between cells, and to be diagnostic markers for cancers. The purpose of this study was to identify circulating miRNAs for predicting the severity of SCI in the acute phase.
Department of Orthopaedic Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.
Mouse SCI models were made using Infinite Horizon impactor with 50 or 70 kdyn compressing power following thoracic laminectomy. The mice were then divided into four groups: normal (without surgery), sham (laminectomy only), mild (50 kdyn), and severe (70 kdyn). TaqMan low-density array analysis and real-time PCR were performed to identify candidate miRNAs that were increased in the serum relative to the severity of SCI.
The expression levels of miR-9*, miR-219 and miR-384-5p in the serum were significantly increased relative to the severity of SCI 12 h after injury. The expression of miR-9* was also significantly increased relative to injury severity at 3 and 24 h after injury.
Serum miR-9*, miR-219 and miR-384-5p might be promising biomarkers for predicting the severity of SCI.
脊髓挫伤小鼠模型的体内研究。
开发一种新指标，用于预测急性脊髓损伤（SCI）的严重程度，并评估新疗法的疗效。据报道，外周血中循环的微小RNA（miRNA）可调节细胞间信号传导，并作为癌症的诊断标志物。本研究的目的是鉴定用于预测急性期SCI严重程度的循环miRNA。
日本广岛大学生物医学与健康科学研究生院骨科。
采用无限视野撞击器，在胸椎椎板切除术后以50或70 kdyn的压缩力制作小鼠SCI模型。然后将小鼠分为四组：正常组（未手术）、假手术组（仅行椎板切除术）、轻度损伤组（50 kdyn）和重度损伤组（70 kdyn）。进行TaqMan低密度阵列分析和实时PCR，以鉴定血清中相对于SCI严重程度增加的候选miRNA。
损伤后12小时，血清中miR-9*、miR-219和miR-384-5p的表达水平相对于SCI严重程度显著增加。损伤后3小时和24小时，miR-9*的表达相对于损伤严重程度也显著增加。
血清miR-9*、miR-219和miR-384-5p可能是预测SCI严重程度的有前景的生物标志物。
