Suppr超能文献

绿脓菌素刺激群体感应介导的对氧化应激的耐受性,并增加鲍曼不动杆菌中的持留菌细胞数量。

Pyocyanin stimulates quorum sensing-mediated tolerance to oxidative stress and increases persister cell populations in Acinetobacter baumannii.

作者信息

Bhargava Nidhi, Sharma Prince, Capalash Neena

机构信息

Department of Biotechnology, Panjab University, Chandigarh, India.

Department of Microbiology, Panjab University, Chandigarh, India.

出版信息

Infect Immun. 2014 Aug;82(8):3417-25. doi: 10.1128/IAI.01600-14. Epub 2014 Jun 2.

Abstract

Acinetobacter baumannii and Pseudomonas aeruginosa are nosocomial pathogens with overlapping sites of infection. This work reports that the two can coexist stably in mixed-culture biofilms. In a study intended to improve our understanding of the mechanism of their coexistence, it was found that pyocyanin, produced by P. aeruginosa that generally eliminates competition from other pathogens, led to the generation of reactive oxygen species (ROS) in A. baumannii cells, which in response showed a significant (P ≤ 0.05) increase in production of enzymes, specifically, catalase and superoxide dismutase (SOD). This work shows for the first time that the expression of catalase and SOD is under the control of a quorum-sensing system in A. baumannii. In support of this observation, a quorum-sensing mutant of A. baumannii (abaI::Km) was found to be sensitive to pyocyanin compared to its wild type and showed significantly (P ≤ 0.001) lower levels of the antioxidant enzymes, which increased on addition of 5 μM N-(3-hydroxydodecanoyl)-l-homoserine lactone. Likewise, in wild-type A. baumannii, there was a significant (P < 0.01) decrease in the level of anti-oxidant enzymes in the presence of salicylic acid, a known quencher of quorum sensing. In the presence of amikacin and carbenicillin, A. baumannii formed 0.07 and 0.02% persister cells, which increased 4- and 3-fold, respectively, in the presence of pyocyanin. These findings show that pyocyanin induces a protective mechanism in A. baumannii against oxidative stress and also increases its persistence against antibiotics which could be of clinical significance in the case of coinfections with A. baumannii and P. aeruginosa.

摘要

鲍曼不动杆菌和铜绿假单胞菌是感染部位重叠的医院病原体。这项研究报告称,二者能够在混合培养生物膜中稳定共存。在一项旨在加深我们对它们共存机制理解的研究中发现,通常会消除其他病原体竞争的铜绿假单胞菌产生的绿脓菌素,会导致鲍曼不动杆菌细胞中产生活性氧(ROS),作为响应,鲍曼不动杆菌细胞中过氧化氢酶和超氧化物歧化酶(SOD)等酶的产量显著增加(P≤0.05)。这项研究首次表明,鲍曼不动杆菌中过氧化氢酶和SOD的表达受群体感应系统调控。为支持这一观察结果,发现鲍曼不动杆菌的群体感应突变体(abaI::Km)与野生型相比对绿脓菌素敏感,且抗氧化酶水平显著降低(P≤0.001),添加5μM N-(3-羟基十二烷酰基)-L-高丝氨酸内酯后抗氧化酶水平升高。同样,在野生型鲍曼不动杆菌中,已知的群体感应淬灭剂水杨酸存在时,抗氧化酶水平显著降低(P<0.01)。在阿米卡星和羧苄青霉素存在的情况下,鲍曼不动杆菌形成0.07%和0.02%的持留菌,在绿脓菌素存在时分别增加4倍和3倍。这些发现表明,绿脓菌素在鲍曼不动杆菌中诱导了一种针对氧化应激的保护机制,还增加了其对抗生素的耐受性,这在鲍曼不动杆菌和铜绿假单胞菌合并感染的情况下可能具有临床意义。

相似文献

4
Mechanistic understanding of cerium oxide nanoparticle-mediated biofilm formation in Pseudomonas aeruginosa.
Environ Sci Pollut Res Int. 2018 Dec;25(34):34765-34776. doi: 10.1007/s11356-018-3418-8. Epub 2018 Oct 15.
7
Attenuation of quorum sensing-mediated virulence of Acinetobacter baumannii by Glycyrrhiza glabra flavonoids.
Future Microbiol. 2015;10(12):1953-68. doi: 10.2217/fmb.15.107. Epub 2015 Nov 19.

引用本文的文献

1
Inter- and intra-bacterial strain diversity remains the "elephant in the (living) room".
NPJ Antimicrob Resist. 2025 Jul 25;3(1):67. doi: 10.1038/s44259-025-00138-8.
3
Accurate Diagnosis of Is Critical to Mitigating Development of Antibiotic Resistance.
Antibiotics (Basel). 2025 May 15;14(5):509. doi: 10.3390/antibiotics14050509.
5
3-Hydroxybenzoic acid inhibits the virulence attributes and disrupts biofilm production in clinical isolates of Acinetobacter baumannii.
Eur J Clin Microbiol Infect Dis. 2025 Mar;44(3):653-669. doi: 10.1007/s10096-024-05009-0. Epub 2024 Dec 30.
6
Antibiofilm activity of exopolysaccharide-mediated ZnO nanoparticle against Pseudomonas aeruginosa biofilm.
Naunyn Schmiedebergs Arch Pharmacol. 2025 May;398(5):5823-5834. doi: 10.1007/s00210-024-03651-1. Epub 2024 Nov 30.
7
Investigating Sulforaphane's anti-virulence and anti-quorum sensing properties against .
Front Pharmacol. 2024 May 21;15:1406653. doi: 10.3389/fphar.2024.1406653. eCollection 2024.
8
biofilm was inhibited by tryptanthrin through disrupting its different stages and genes expression.
iScience. 2024 May 8;27(6):109942. doi: 10.1016/j.isci.2024.109942. eCollection 2024 Jun 21.
9
The impact of phage and phage resistance on microbial community dynamics.
PLoS Biol. 2024 Apr 22;22(4):e3002346. doi: 10.1371/journal.pbio.3002346. eCollection 2024 Apr.
10
A biomedical perspective of pyocyanin from Pseudomonas aeruginosa: its applications and challenges.
World J Microbiol Biotechnol. 2024 Feb 10;40(3):90. doi: 10.1007/s11274-024-03889-0.

本文引用的文献

1
Heterogeneous persister cells formation in Acinetobacter baumannii.
PLoS One. 2013 Dec 31;8(12):e84361. doi: 10.1371/journal.pone.0084361. eCollection 2013.
2
Stress responses in the opportunistic pathogen Acinetobacter baumannii.
Future Microbiol. 2013 Mar;8(3):353-65. doi: 10.2217/fmb.12.150.
4
5
Toxin-antitoxin systems influence biofilm and persister cell formation and the general stress response.
Appl Environ Microbiol. 2011 Aug 15;77(16):5577-83. doi: 10.1128/AEM.05068-11. Epub 2011 Jun 17.
6
Comparing the microbiota of the cystic fibrosis lung and human gut.
Gut Microbes. 2010 Mar;1(2):85-93. doi: 10.4161/gmic.1.2.11350. Epub 2010 Jan 29.
7
UV-resistant Acinetobacter sp. isolates from Andean wetlands display high catalase activity.
FEMS Microbiol Lett. 2011 Apr;317(2):181-9. doi: 10.1111/j.1574-6968.2011.02231.x. Epub 2011 Mar 2.
8
A simple colorimetric method for testing antimicrobial susceptibility of biofilmed bacteria.
J Microbiol. 2010 Oct;48(5):709-11. doi: 10.1007/s12275-010-0299-z. Epub 2010 Nov 3.
10
Multidrug resistant Acinetobacter baumannii: a descriptive study in a city hospital.
BMC Infect Dis. 2010 Jul 7;10:196. doi: 10.1186/1471-2334-10-196.

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验