用于评估奶牛黄体状态的商用孕酮检测方法比较
Comparison of commercial progesterone assays for evaluation of luteal status in dairy cows.
作者信息
Broes André, LeBlanc Stephen J
机构信息
Biovet, Saint-Hyacinthe, Quebec J2S 8W2 (Broes); Department of Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1 (LeBlanc).
出版信息
Can Vet J. 2014 Jun;55(6):582-4.
Abstract
Serum progesterone (P4) concentration was measured using 2 methods, and ovaries were examined by ultrasonography (US) to determine the presence of a corpus luteum (CL) in 101 lactating dairy cows. The concordance correlation coefficient between the 2 methods (Ovucheck and Immulite P4 assays) was high (ρc = 0.94); agreement between the assays on presence of a CL based on P4 > 1 ng/mL was excellent (Kappa = 0.88) and of each assay with US was good (Kappa = 0.63 for each).
摘要
采用两种方法测定了101头泌乳奶牛的血清孕酮（P4）浓度，并通过超声检查（US）检查卵巢以确定黄体（CL）的存在。两种方法（Ovucheck和免疫发光法P4检测）之间的一致性相关系数很高（ρc = 0.94）；基于P4>1 ng/mL的CL存在情况检测之间的一致性极佳（Kappa = 0.88），且每种检测方法与超声检查的一致性良好（每种方法的Kappa = 0.63）。
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