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Mitochondria of the yeasts Saccharomyces cerevisiae and Kluyveromyces lactis contain nuclear rDNA-encoded proteins.酵母酿酒酵母和乳酸克鲁维酵母的线粒体含有核 rDNA 编码的蛋白质。
PLoS One. 2011 Jan 25;6(1):e16325. doi: 10.1371/journal.pone.0016325.
3
Superoxide radical induces sclerotial differentiation in filamentous phytopathogenic fungi: a superoxide dismutase mimetics study.超氧自由基诱导丝状植物病原真菌产生菌核分化：超氧化物歧化酶模拟物研究。
Microbiology (Reading). 2010 Mar;156(Pt 3):960-966. doi: 10.1099/mic.0.034579-0. Epub 2009 Dec 10.
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Differential gene expression in the murine gastric fundus lacking interstitial cells of Cajal.缺乏 Cajal 间质细胞的小鼠胃底中的差异基因表达
BMC Gastroenterol. 2003 Jun 10;3:14. doi: 10.1186/1471-230X-3-14.
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Distribution and cloning of eukaryotic mRNAs by means of differential display: refinements and optimization.通过差异显示进行真核生物mRNA的分布与克隆：改进与优化
Nucleic Acids Res. 1993 Jul 11;21(14):3269-75. doi: 10.1093/nar/21.14.3269.
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Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction.通过聚合酶链反应对真核生物信使核糖核酸进行差异显示
Science. 1992 Aug 14;257(5072):967-71. doi: 10.1126/science.1354393.

菌核形成期羊肚菌基因表达差异。

Gene Expressing Difference in Sclerotial Formation of Morchella conica.

机构信息

College of Life Sciences, Yunnan University, Kunming, 650091 Yunnan People's Republic of China ; Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming, 650221 Yunnan People's Republic of China.

Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming, 650221 Yunnan People's Republic of China ; Yunnan Provincial Key Lab of Agricultural Biotechnology, Kunming, 650221 Yunnan People's Republic of China ; Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture, 9 Xueyun Road, Wuhua, Kunming, 650221 Yunnan People's Republic of China.

出版信息

Indian J Microbiol. 2014 Sep;54(3):274-83. doi: 10.1007/s12088-014-0445-0. Epub 2014 Jan 16.

DOI:10.1007/s12088-014-0445-0
PMID:24891734
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4039721/
Abstract

The difference of gene expression between sclerotia-producing and non-sclerotia-producing single spore isolates from Morchella conica were preliminary analyzed by mRNA differential display reverse transcription-polymerase chain reaction (RT-PCR) technique and 67 differential gene fragments were obtained. Fifty-eight of their second PCR products were cloned and sequenced. Thirteen special differential gene fragments related to sclerotial formation were validated by semi-quantitative RT-PCR. Some gene fragments had certain homologies with lipoprotein, cyclin-dependent kinase C-3, glycerophosphoryl diester phosphodiesterase, Rho GDP-dissociation inhibitor, gamma-aminobutyrate permease, OmpA family protein, Transcript antisense to ribosomal RNA protein, sodium-calcium exchange protein and keratin-associated proteins 5, 6. In addition, the putative protein of some DNA fragments had higher similarity with hypothetical protein-coding gene in NCBI database, as well as some were only putative gene fragments. All these fragments were speculated to be the functional gene associated with sclerotial formation in morel.

摘要

通过 mRNA 差异显示反转录-聚合酶链反应（RT-PCR）技术初步分析了来自羊肚菌的产菌核和不产菌核单孢分离物之间的基因表达差异，获得了 67 个差异基因片段。其中 58 个片段的第二次 PCR 产物被克隆并测序。通过半定量 RT-PCR 验证了与菌核形成相关的 13 个特殊差异基因片段。一些基因片段与脂蛋白、细胞周期蛋白依赖性激酶 C-3、甘油磷酸二酯磷酸二酯酶、Rho GDP 解离抑制剂、γ-氨基丁酸通透酶、OmpA 家族蛋白、核糖体 RNA 蛋白反义转录物、钠钙交换蛋白和角蛋白相关蛋白 5、6 具有一定的同源性。此外，一些 DNA 片段的假定蛋白与 NCBI 数据库中的假定蛋白编码基因具有更高的相似性，而有些则只是假定基因片段。所有这些片段都被推测为与羊肚菌菌核形成相关的功能基因。