Wall R Andrew, Barton Jennifer K
University of Arizona, College of Optical Sciences, 1630 E. University Boulevard, Tucson, Arizona 85721.
University of Arizona, College of Optical Sciences, 1630 E. University Boulevard, Tucson, Arizona 85721bUniversity of Arizona, Department of Biomedical Engineering, 1127 E. James E. Rogers Way, Tucson, Arizona 85721.
J Biomed Opt. 2014 Jun;19(6):067002. doi: 10.1117/1.JBO.19.6.067002.
A side-viewing, 2.3-mm diameter oblique incidence reflectometry endoscope has been designed to obtain optical property measurements of turbid samples. Light from a single-mode fiber is relayed obliquely onto the tissue with a gradient index lens-based distal optics assembly and the resulting diffuse reflectance profile is imaged and collected with a 30,000 element, 0.72 mm clear aperture fiber bundle. Sampling the diffuse reflectance in two-dimensions allows for fitting of the reflected intensity profile to a well-known theoretical model, permitting the extraction of both absorption and reduced scattering coefficients of the tissue sample. Models and measurements of the endoscopic imaging system are presented in tissue phantoms and in vivo mouse colon, verifying the endoscope's capabilities to accurately measure effective attenuation coefficient and differentiate diseased from normal colon.
一种直径为2.3毫米的侧视斜入射反射测量内窥镜已被设计用于获取浑浊样本的光学特性测量值。来自单模光纤的光通过基于梯度折射率透镜的远端光学组件斜向中继到组织上，然后将由此产生的漫反射轮廓成像并用一个由30000个元件、孔径为0.72毫米的透明光纤束进行收集。在二维空间中对漫反射进行采样，可以将反射强度轮廓拟合到一个著名的理论模型，从而能够提取组织样本的吸收系数和约化散射系数。本文展示了在内窥镜成像系统在组织模型和活体小鼠结肠中的模型和测量结果，验证了该内窥镜准确测量有效衰减系数以及区分病变结肠和正常结肠的能力。
