Muzzio N E, Pasquale M A, González P H, Arvia A J
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), Universidad Nacional de La Plata (UNLP), CONICET, Sucursal 4, Casilla de Correo 16, 1900, La Plata, Argentina.
J Biol Phys. 2014 Jun;40(3):285-308. doi: 10.1007/s10867-014-9349-9. Epub 2014 Jun 4.
The dynamics of in situ 2D HeLa cell quasi-linear and quasi-radial colony fronts in a standard culture medium is investigated. For quasi-radial colonies, as the cell population increased, a kinetic transition from an exponential to a constant front average velocity regime was observed. Special attention was paid to individual cell motility evolution under constant average colony front velocity looking for its impact on the dynamics of the 2D colony front roughness. From the directionalities and velocity components of cell trajectories in colonies with different cell populations, the influence of both local cell density and cell crowding effects on individual cell motility was determined. The average dynamic behaviour of individual cells in the colony and its dependence on both local spatio-temporal heterogeneities and growth geometry suggested that cell motion undergoes under a concerted cell migration mechanism, in which both a limiting random walk-like and a limiting ballistic-like contribution were involved. These results were interesting to infer how biased cell trajectories influenced both the 2D colony spreading dynamics and the front roughness characteristics by local biased contributions to individual cell motion. These data are consistent with previous experimental and theoretical cell colony spreading data and provide additional evidence of the validity of the Kardar-Parisi-Zhang equation, within a certain range of time and colony front size, for describing the dynamics of 2D colony front roughness.
研究了标准培养基中原位二维HeLa细胞准线性和准径向集落前沿的动力学。对于准径向集落，随着细胞数量的增加，观察到前沿平均速度从指数型到恒定型的动力学转变。在集落前沿平均速度恒定的情况下，特别关注单个细胞运动性的演变，以寻找其对二维集落前沿粗糙度动力学的影响。从不同细胞数量的集落中细胞轨迹的方向性和速度分量，确定了局部细胞密度和细胞拥挤效应对单个细胞运动性的影响。集落中单个细胞的平均动态行为及其对局部时空异质性和生长几何形状的依赖性表明，细胞运动是在一种协同细胞迁移机制下进行的，其中涉及到类似极限随机游走和类似极限弹道的贡献。这些结果有助于推断有偏的细胞轨迹如何通过对单个细胞运动的局部有偏贡献来影响二维集落扩展动力学和前沿粗糙度特征。这些数据与先前的实验和理论细胞集落扩展数据一致，并在一定的时间和集落前沿大小范围内，为Kardar-Parisi-Zhang方程描述二维集落前沿粗糙度动力学的有效性提供了额外证据。
