Mandal Santi M, Roy Anupam, Mahata Denial, Migliolo Ludovico, Nolasco Diego O, Franco Octavio L
Mass Spectrometry & Proteomics Lab, Central Research Facility, Indian Institute of Technology, Kharagpur, Kharagpur, India.
Centro de Análises Proteômicas e Bioquímicas, Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, UCB, Brasília, Brazil.
J Antibiot (Tokyo). 2014 Nov;67(11):771-5. doi: 10.1038/ja.2014.70. Epub 2014 Jun 4.
A novel antibacterial ointment using bacitracin, specific for Gram-positive bacteria, and gramicidin S, a highly toxic antibacterial peptide, was here developed showing broad-spectrum antibacterial activities against pathogenic strains with less toxicity after self-assembly into nanofiber structures. Such structures were confirmed with scanning electron microscopy and CD analyses. In addition, in silico studies using docking associated with molecular dynamics were carried out to obtain information about fiber structural oligomerization. Thus, the bacitracin and gramicidin S-based self-assembled nanopeptide ribbon may be a successful ointment formulation for bacterial infection control.
一种新型抗菌软膏在此被研发出来，它使用对革兰氏阳性菌有特异性的杆菌肽以及一种剧毒抗菌肽短杆菌肽S，在自组装成纳米纤维结构后，对致病菌株显示出广谱抗菌活性且毒性较小。通过扫描电子显微镜和圆二色性分析证实了此类结构。此外，还进行了使用对接结合分子动力学的计算机模拟研究，以获取有关纤维结构寡聚化的信息。因此，基于杆菌肽和短杆菌肽S的自组装纳米肽带可能是一种成功的用于控制细菌感染的软膏制剂。
