细胞内压力的直接测量。
Direct measurement of intracellular pressure.
作者信息
Petrie Ryan J, Koo Hyun
机构信息
Laboratory of Cell and Developmental Biology, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland.
出版信息
Curr Protoc Cell Biol. 2014 Jun 3;63:12.9.1-9. doi: 10.1002/0471143030.cb1209s63.
Abstract
A method to directly measure the intracellular pressure of adherent, migrating cells is described in this unit. This approach is based on the servo-null method where a microelectrode is introduced into the cell to directly measure the physical pressure of the cytoplasm. We also describe the initial calibration of the microelectrode, as well as the application of the method to cells migrating inside three-dimensional (3-D) extracellular matrix (ECM).
摘要
本单元介绍了一种直接测量贴壁迁移细胞胞内压力的方法。该方法基于伺服零位法，即将微电极插入细胞以直接测量细胞质的物理压力。我们还描述了微电极的初始校准，以及该方法在三维（3-D）细胞外基质（ECM）中迁移的细胞上的应用。
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本文引用的文献
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