Dinescu Sorina, Galateanu Bianca, Lungu Adriana, Radu Eugen, Nae Sorin, Iovu Horia, Costache Marieta
Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independentei, 050095 Bucharest, Romania.
Advanced Polymer Materials Group, Department of Bioresources and Polymer Science, University Politehnica of Bucharest, 149 Calea Victoriei, 010072 Bucharest, Romania.
Biomed Res Int. 2014;2014:830791. doi: 10.1155/2014/830791. Epub 2014 May 4.
Recent progress in tissue engineering and regenerative medicine envisages the use of cell-scaffold bioconstructs to best mimic the natural in vivo microenvironment. Our aim was not only to develop novel 3D porous scaffolds for regenerative applications by the association of gelatin (G), alginate (A), and polyacrylamide (PAA) major assets but also to evaluate their in vitro potential to support human adipose-derived stem cells (hADSCs) adipogenesis. G-A-PAA biomatrix investigated in this work is an interesting substrate combining the advantages of the three individual constituents, namely, biodegradability of G, hydrophilicity of A and PAA, superior elasticity at compression with respect to the G-A and PAA controls, and the capacity to generate porous scaffolds. hADSCs inside these novel interpenetrating polymer networks (IPNs) were able to populate the entire scaffold structure and to display their characteristic spindle-like shape as a consequence of a good interaction with G component of the matrices. Additionally, hADSCs proved to display the capacity to differentiate towards mature adipocytes, to accumulate lipids inside their cytoplasm, and to express perilipin late adipogenic marker inside novel IPNs described in this study. On long term, this newly designed biomatrix aims to represent a stem cell delivery system product dedicated for modern regenerative strategies.
组织工程和再生医学的最新进展设想使用细胞-支架生物构建体来最佳地模拟天然的体内微环境。我们的目标不仅是通过结合明胶（G）、藻酸盐（A）和聚丙烯酰胺（PAA）的主要特性来开发用于再生应用的新型三维多孔支架，还评估它们在体外支持人脂肪来源干细胞（hADSCs）脂肪生成的潜力。在这项工作中研究的G-A-PAA生物基质是一种有趣的基质，它结合了三种单独成分的优点，即G的生物可降解性、A和PAA的亲水性、相对于G-A和PAA对照在压缩时具有优异的弹性以及生成多孔支架的能力。这些新型互穿聚合物网络（IPNs）中的hADSCs能够填充整个支架结构，并由于与基质的G成分良好相互作用而呈现其特征性的纺锤状形状。此外，hADSCs被证明具有向成熟脂肪细胞分化的能力，能够在其细胞质内积累脂质，并在本研究中描述的新型IPNs内表达脂肪生成晚期标记物围脂滴蛋白。从长远来看，这种新设计的生物基质旨在成为一种专门用于现代再生策略的干细胞递送系统产品。
