Dinescu Sorina, Galateanu Bianca, Albu Madalina, Cimpean Anisoara, Dinischiotu Anca, Costache Marieta
Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independentei, Bucharest 050095, Romania.
Int J Mol Sci. 2013 Jan 16;14(1):1870-89. doi: 10.3390/ijms14011870.
Current clinical strategies for adipose tissue engineering (ATE), including autologous fat implants or the use of synthetic surrogates, not only are failing in the long term, but also can't face the latest requirements regarding the aesthetic restoration of the resulted imperfections. In this context, modern strategies in current ATE applications are based on the implantation of 3D cell-scaffold bioconstructs, designed for prospective achievement of in situ functional de novo tissue. Thus, in this paper, we reported for the first time the evaluation of a spongious 60% collagen and 40% sericin scaffold preseeded with human adipose-derived stem cells (hADSCs) in terms of biocompatibility and adipogenic potential in vitro. We showed that the addition of the sticky protein sericin in the composition of a classical collagen sponge enhanced the adhesion and also the proliferation rate of the seeded cells, thus improving the biocompatibility of the novel scaffold. In addition, sericin stimulated PPARγ2 overexpression, triggering a subsequent upregulated expression profile of FAS, aP2 and perilipin adipogenic markers. These features, together with the already known sericin stimulatory potential on cellular collagen production, promote collagen-sericin biomatrix as a good candidate for soft tissue reconstruction and wound healing applications.
当前脂肪组织工程（ATE）的临床策略，包括自体脂肪植入或使用合成替代物，不仅长期效果不佳，而且无法满足对修复后出现的瑕疵进行美学修复的最新要求。在此背景下，当前ATE应用中的现代策略基于植入3D细胞支架生物构建体，旨在预期实现原位功能性新生组织。因此，在本文中，我们首次报道了对预先接种人脂肪来源干细胞（hADSCs）的60%胶原蛋白和40%丝胶蛋白海绵支架进行体外生物相容性和成脂潜力评估。我们发现，在经典胶原海绵成分中添加粘性蛋白丝胶蛋白可增强接种细胞的黏附力和增殖率，从而提高新型支架的生物相容性。此外，丝胶蛋白刺激PPARγ2过表达，引发随后脂肪酸合酶（FAS）、脂肪细胞脂肪酸结合蛋白（aP2）和脂肪分化相关蛋白（perilipin）成脂标志物表达谱上调。这些特性，连同丝胶蛋白对细胞胶原蛋白产生的已知刺激潜力，使胶原-丝胶蛋白生物基质成为软组织重建和伤口愈合应用的良好候选材料。
