Nishizawa Masako, Seshimo Akiyoshi, Miyake Kunitomo, Amano Kunihiko, Kameoka Shingo
Hepatogastroenterology. 2014 Jan-Feb;61(129):240-4.
Peritoneal dissemination from gastric cancer is the most frequent metastasis of advanced gastric cancer. Detection of cancer micrometastasis is required for improvement of cancer therapy. A method that can detect micrometastases more sensitively is desired. Against this background, for the purposes of making the genetic diagnosis of micrometastasis simple and rapid, TRC (transcription reverse transcription concerted reaction) was developed.
69 patients with gastric cancer in those diagnosed with deeper than mp. At the time of surgery, peritoneal washing with saline was extracted. Having extracted the RNA therein, It was blended with a reagent responsive to CEAmRNA and was put into TRC. The cytology and TRC were compared and studied.
The concordance rate between TRC and cytology was K=0.6552. The patients whom there was a discrepancy between the cytology and TRC are clinically validate the TRC results. In the study of prognosis, TRC obtained a sensitivity of 90.9% and a specificity of 98.3%.
The measurement of CEAmRNA in peritoneal washing specimens by the TRC method is superior to Cytology. That is also equal to or superior to the RT-PCR method with respect to sensitivity and specificity, and superior with respect to simplicity and rapidity.
胃癌腹膜播散是进展期胃癌最常见的转移途径。为改善癌症治疗效果，需要检测癌症微转移。人们期望有一种能更灵敏地检测微转移的方法。在此背景下，为了使微转移的基因诊断简单快速，开发了TRC（转录逆转录协同反应）技术。
选取69例胃癌侵犯深度超过黏膜下层（mp）的患者。手术时，用生理盐水冲洗腹腔并提取冲洗液。提取其中的RNA，与针对癌胚抗原信使核糖核酸（CEAmRNA）的试剂混合后进行TRC检测。对细胞学检查结果与TRC检测结果进行比较研究。
TRC与细胞学检查的一致性率为K = 0.6552。细胞学检查结果与TRC检测结果存在差异的患者，经临床验证，TRC结果有效。在预后研究中，TRC的灵敏度为90.9%，特异度为98.3%。
采用TRC技术检测腹腔冲洗液标本中的CEAmRNA，优于细胞学检查。在灵敏度和特异度方面，该技术与逆转录聚合酶链反应（RT-PCR）技术相当或更优，且在操作简便性和检测速度方面更具优势。
