State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China.
Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, PR China.
Int J Syst Evol Microbiol. 2014 Sep;64(Pt 9):2956-2961. doi: 10.1099/ijs.0.061739-0. Epub 2014 Jun 4.
A Gram-stain-negative, facultatively anaerobic, non-motile and coccoid- to short-rod-shaped bacterium, designated strain Dys-CH1(T), was isolated from the hindgut of a fungus-growing termite Macrotermes barneyi. The optimal pH and cultivation temperature of strain Dys-CH1(T) were pH 7.2-7.6 and 35-37 °C, respectively. Sequence analysis of 16S rRNA gene showed that Dys-CH1(T) shared 94.6 % and 90.9 % similarity with Dysgonomonas capnocytophagoides JCM 16697(T) and Dysgonomonas gadei CCUG 42882(T), respectively. Strain Dys-CH1(T) was found to be different from other species of the genus Dysgonomonas with validly published names with respect to taxonomically important traits, including habitat, biochemical tests, DNA G+C content, bile resistance, fatty-acid composition and susceptibility to antimicrobial agents. On the basis of these characteristics, strain Dys-CH1(T) represents a novel species of the genus Dysgonomonas for which the name Dysgonomonas macrotermitis sp. nov. is proposed. The type strain is Dys-CH1(T) ( = JCM 19375(T) = DSM 27370(T)).
一株革兰氏阴性、兼性厌氧、非运动和球形到短杆形的细菌，被命名为 Dys-CH1(T) 菌株，从真菌生长的白蚁 Macrotermes barneyi 的后肠中分离出来。Dys-CH1(T) 菌株的最佳 pH 值和培养温度分别为 pH7.2-7.6 和 35-37°C。16S rRNA 基因序列分析表明，Dys-CH1(T) 与 Dysgonomonas capnocytophagoides JCM 16697(T) 和 Dysgonomonas gadei CCUG 42882(T) 的相似度分别为 94.6%和 90.9%。Dys-CH1(T) 菌株与其他已发表名称的属 Dysgonomonas 种在分类学上重要的特征上有所不同，包括栖息地、生化试验、DNA G+C 含量、胆汁抗性、脂肪酸组成和对抗菌药物的敏感性。基于这些特征，Dys-CH1(T) 菌株代表了 Dysgonomonas 属的一个新种，建议将其命名为 Dysgonomonas macrotermitis sp. nov.。该模式菌株为 Dys-CH1(T)（=JCM 19375(T)=DSM 27370(T)）。
