脂肪来源干细胞在严重急性碱性烧伤动物模型的巩膜接触镜载体上的应用
Application of adipose-derived stem cells on scleral contact lens carrier in an animal model of severe acute alkaline burn.
作者信息
Espandar Ladan, Caldwell Delmar, Watson Richard, Blanco-Mezquita Tomas, Zhang Shijia, Bunnell Bruce
机构信息
Department of Ophthalmology (L.E.), Duke University Eye Center, Durham, NC; Department of Ophthalmology (D.C., R.W.), School of Medicine, Tulane University, New Orleans, LA; Instituto Oftalmobiologia Aplicada (T.B-M.), University of Valladolid, Valladolid, Spain; Center for Stem Cell Research and Regenerative Medicine (S.Z., B.B.), New Orleans, LA; Department of Pharmacology (S.Z., B.B.), School of Medicine, Tulane University, New Orleans, LA; and Division of Regenerative Medicine (B.B.), Tulane National Primate Research Center, Covington, LA.
出版信息
Eye Contact Lens. 2014 Jul;40(4):243-7. doi: 10.1097/ICL.0000000000000045.
PURPOSE
To evaluate the therapeutic effect of human adipose-derived stem cells (hASCs) overlaid on a scleral contact lens (SCL) carrier in a rabbit model of ocular alkaline burn.
MATERIALS AND METHODS
After inducing alkaline burn in 11 New Zealand white rabbits, hASCs cultured on SCLs were placed on the right eye of 5 rabbits, SCLs without cells were used in 5, and no treatment was applied in 1 eye. Each eye was examined and photographed for corneal vascularization, opacities, and epithelial defect in week 1, 2, and 4 after surgery. After 1 month, rabbits were killed and the corneas were removed and cut in half for electron and light microscopy examination.
RESULTS
Human adipose-derived stem cells were attached to SCL surface and confluent easily. Human adipose-derived stem cells on SCL eyes showed smaller epithelial defect, less corneal opacity, corneal neovascularization relative to SCL eyes. Both groups showed no symblepharon. However, the cornea in the untreated eye was melted in 2 weeks and developed severe symblepharon.
CONCLUSION
Human adipose-derived stem cells on SCL can reduce inflammation and corneal haziness in severe ocular alkaline burn injury in rabbits.
目的
在兔眼碱性烧伤模型中评估覆盖在巩膜接触镜（SCL）载体上的人脂肪来源干细胞（hASCs）的治疗效果。
材料与方法
对11只新西兰白兔诱导碱性烧伤后，将培养在SCL上的hASCs置于5只兔子的右眼，5只兔子使用无细胞的SCL，1只眼睛不进行治疗。在术后第1、2和4周对每只眼睛进行检查并拍摄角膜血管化、混浊和上皮缺损情况。1个月后，处死兔子，取出角膜并切成两半进行电子显微镜和光学显微镜检查。
结果
人脂肪来源干细胞附着于SCL表面且易于汇合。与使用SCL的眼睛相比，使用SCL并接种hASCs的眼睛上皮缺损更小，角膜混浊和角膜新生血管更少。两组均未出现睑球粘连。然而，未治疗眼睛的角膜在2周内溶解并出现严重睑球粘连。
结论
SCL上的人脂肪来源干细胞可减轻兔眼严重碱性烧伤损伤中的炎症和角膜混浊。
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