Camarda Massimo, Fisicaro Giuseppe, Anzalone Ruggero, Scalese Silvia, Alberti Alessandra, La Via Francesco, La Magna Antonino, Ballo Andrea, Giustolisi Gianluca, Minafra Luigi, Cammarata Francesco P, Bravatà Valentina, Forte Giusi I, Russo Giorgio, Gilardi Maria C
CNR-IMM Sezione di Catania, Z,I, VIII Strada 5, I-95121 Catania, Italy.
Biomed Eng Online. 2014 Jun 5;13:71. doi: 10.1186/1475-925X-13-71.
We aimed to investigate the effect of cell-cell dipole interactions in the equilibrium distributions in dielectrophoretic devices.
We used a three dimensional coupled Monte Carlo-Poisson method to theoretically study the final distribution of a system of uncharged polarizable particles suspended in a static liquid medium under the action of an oscillating non-uniform electric field generated by polynomial electrodes. The simulated distributions have been compared with experimental ones observed in the case of MDA-MB-231 cells in the same operating conditions.
The real and simulated distributions are consistent. In both cases the cells distribution near the electrodes is dominated by cell-cell dipole interactions which generate long chains.
The agreement between real and simulated cells' distributions demonstrate the method's reliability. The distribution are dominated by cell-cell dipole interactions even at low density regimes (105 cell/ml). An improved estimate for the density threshold governing the interaction free regime is suggested.
我们旨在研究细胞 - 细胞偶极相互作用对介电泳装置中平衡分布的影响。
我们使用三维耦合蒙特卡罗 - 泊松方法，从理论上研究悬浮在静态液体介质中的不带电可极化粒子系统在由多项式电极产生的振荡非均匀电场作用下的最终分布。将模拟分布与在相同操作条件下观察到的MDA - MB - 231细胞的实验分布进行了比较。
实际分布与模拟分布一致。在这两种情况下，电极附近的细胞分布都由产生长链的细胞 - 细胞偶极相互作用主导。
实际细胞分布与模拟细胞分布之间的一致性证明了该方法的可靠性。即使在低密度状态（105个细胞/毫升）下，分布也由细胞 - 细胞偶极相互作用主导。提出了对控制相互作用自由状态的密度阈值的改进估计。
