Bolarinwa Islamiyat F, Orfila Caroline, Morgan Michael R A
School of Food Science and Nutrition, University of Leeds , Leeds LS2 9JT, United Kingdom.
J Agric Food Chem. 2014 Jul 9;62(27):6299-305. doi: 10.1021/jf501978d. Epub 2014 Jun 26.
Amygdalin is a member of the cyanogenic glycoside group of plant secondary metabolites capable of generating hydrogen cyanide under certain conditions. As a consequence, the cyanogenic glycosides have been associated with incidents of acute and subacute food poisoning. Specific antibodies were raised against an amygdalin-bovine serum albumin immunogen synthesized using a novel approach. The antibodies were used in a microtitration plate enzyme-linked immunosorbent assay (ELISA) for the quantification, for the first time, of amygdalin in commercially available foods. Correlation of results with high-performance liquid chromatography was very high (r = 0.983). The limit of detection of the immunoassay was 200 ± 0.05 pg mL(-1), and the 50% inhibitory concentration of amygdalin was 50 ± 0.02 ng mL(-1), making the ELISA particularly sensitive.
苦杏仁苷是植物次生代谢产物中氰苷类的一员，在某些条件下能够产生氰化氢。因此，氰苷类与急性和亚急性食物中毒事件有关。针对采用新方法合成的苦杏仁苷-牛血清白蛋白免疫原制备了特异性抗体。这些抗体首次用于微量滴定板酶联免疫吸附测定（ELISA）中，以定量市售食品中的苦杏仁苷。结果与高效液相色谱法的相关性非常高（r = 0.983）。免疫测定的检测限为200±0.05 pg mL⁻¹，苦杏仁苷的50%抑制浓度为50±0.02 ng mL⁻¹，这使得ELISA特别灵敏。
