Cho A-Yeon, Shin Kum-Joo, Chung Junho, Oh Sangsuk
Department of Biochemistry and Molecular Biology, Seoul National University College of Medicine, Seoul 110-799, South Korea.
J Food Prot. 2008 Oct;71(10):2048-52. doi: 10.4315/0362-028x-71.10.2048.
Amygdalin (laterile) is a cyanogenic glycoside commonly found in the pits of many fruits and raw nuts. When amygdalin-containing seeds are crushed and moistened, free cyanide is formed. Pits and nuts containing unusually high levels of amygdalin can therefore cause cyanide poisoning, and detection of amygdalin in food extracts can be a life-saving measure. In this study, we generated recombinant antibodies against amygdalin from a phage display of a combinatorial rabbit/human chimeric antibody library and used it in a sensitive competition enzyme immunoassay system to detect amygdalin in extracts of pits and nuts. The detection limit was determined to be 1 x 10(-9) M.
苦杏仁苷（扁桃苷）是一种常见于许多水果核和生坚果中的含氰糖苷。当含有苦杏仁苷的种子被碾碎并受潮时，会形成游离氰化物。因此，含有异常高含量苦杏仁苷的果核和坚果会导致氰化物中毒，而检测食品提取物中的苦杏仁苷可能是一项挽救生命的措施。在本研究中，我们从组合兔/人嵌合抗体文库的噬菌体展示中产生了针对苦杏仁苷的重组抗体，并将其用于灵敏的竞争酶免疫分析系统，以检测果核和坚果提取物中的苦杏仁苷。检测限确定为1×10⁻⁹ M。
