INGIbio Co. Ltd., R&D Center, 206, APRI, 123 Chemdan-gwagiro, Buk-gu, Gwangju 500-712, Republic of Korea.
School of Physics and Chemistry, Gwangju Institute of Science and Technology, Gwangju 500-712, Republic of Korea.
Biosens Bioelectron. 2014 Nov 15;61:285-9. doi: 10.1016/j.bios.2014.04.032. Epub 2014 May 14.
The lateral flow assay (LFA) strip sensor possesses many advantages as a diagnostic device, including the capabilities of rapid, one-step assay performance, and high throughput production. A major limitation of the sensor, however, is its difficulty in measuring a broad concentration range of target proteins, including C-reactive protein (CRP), due to the "hook effect." In this study, we report the use of a three-line LFA strip sensor, adding an antigen line to the conventional two-line LFA sensor, for detecting CRP within a broad concentration range in human sera. We introduced an antigen line between test and control lines in the LFA sensor. The antigen line was formed by dispensing a CRP antibody solution followed by a CRP solution in nitrocellulose membrane. All other conditions were identical to those applied to the conventional LFA strip sensor. The CRP level in test samples was generated by data processing from the intensities of three lines. The strip sensor measured a linear detection range of CRP concentration from 1 ng/mL to 500 μg/mL within 10 min, with a calculated detection range of 0.69 ng/mL-1.02 mg/mL. Using the developed three-line LFA sensor, 50 clinical samples were measured at a detection range of 0.4-84.7 μg/mL. This novel and easy-to-use CRP sensor can be a useful tool for rapid, sensitive, and cost-effective detection of a broad physiological concentration range of CRP capabilities that are vital for various diagnostic applications.
侧向流动检测（LFA）条传感器作为一种诊断设备具有许多优点，包括快速、一步检测性能和高通量生产能力。然而，传感器的一个主要限制是由于“钩状效应”，难以测量广泛浓度范围的目标蛋白，包括 C 反应蛋白（CRP）。在本研究中，我们报告了使用三线 LFA 条传感器，在传统的二线 LFA 传感器中添加抗原线，用于检测人血清中 CRP 的广泛浓度范围。我们在 LFA 传感器的测试线和控制线之间引入了抗原线。抗原线是通过在硝酸纤维素膜上分配 CRP 抗体溶液，然后分配 CRP 溶液形成的。所有其他条件均与传统 LFA 条传感器相同。测试样品中的 CRP 水平通过对三条线的强度进行数据处理生成。该条传感器在 10 分钟内测量 CRP 浓度的线性检测范围为 1ng/mL 至 500μg/mL，计算的检测范围为 0.69ng/mL-1.02mg/mL。使用开发的三线 LFA 传感器，在 0.4-84.7μg/mL 的检测范围内测量了 50 个临床样本。这种新型且易于使用的 CRP 传感器可以成为快速、灵敏和具有成本效益的广泛生理浓度范围 CRP 检测的有用工具，这对于各种诊断应用至关重要。
