Meng Fanda, Zhang Lei, Huo Weisong, Lian Jie, Jesorka Aldo, Shi Xizeng, Gao Yunhua
Department of Clinical Laboratory Medicine, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Shandong Medicine and Health Key Laboratory of Laboratory Medicine, Jinan 250014, China.
School of Basic Medicine, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250062, China.
ACS Omega. 2021 May 7;6(19):12923-12930. doi: 10.1021/acsomega.1c01603. eCollection 2021 May 18.
In this study, we report a convenient analytical method for a full-range quantification of the C-reactive protein (CRP), a blood biomarker of infection and cardiovascular events. We determine CRP over the entire diagnostically relevant concentration range in undiluted human blood serum in a single test, using a tandem giant magnetoresistance (GMR) sensor. The tandem principle combines a sandwich assay and a competitive assay, which allows for the discrimination of the concentration values resulting from the multivalued dose-response curve ("Hook" effect), which characterizes the one-step sandwich assay at high CRP concentrations. The sensor covers a linear detection range for CRP concentration from 3 ng/mL to 350 μg/mL, the detection limit (s/n = 3) is 1 ng/mL. The prominent features of the chip-based method are its expanded dynamic range and low sample volume (50 μL), and the need for a short measurement time of 15 min. These figures of merit, in addition to the low detection limit equal to the established assay instrumentation, make it a viable candidate for use in point-of-care diagnostics.
在本研究中，我们报告了一种便捷的分析方法，用于对C反应蛋白（CRP）进行全范围定量分析，CRP是感染和心血管事件的血液生物标志物。我们使用串联巨磁电阻（GMR）传感器，在单次测试中对未稀释的人血清中整个诊断相关浓度范围内的CRP进行测定。串联原理结合了夹心测定法和竞争测定法，这使得能够区分由多值剂量反应曲线（“钩状”效应）产生的浓度值，该效应在高CRP浓度下表征一步夹心测定法。该传感器对CRP浓度的线性检测范围为3 ng/mL至350 μg/mL，检测限（信噪比=3）为1 ng/mL。基于芯片的方法的突出特点是其扩展的动态范围和低样品体积（50 μL），以及测量时间短至15分钟的要求。除了与既定检测仪器相当的低检测限外，这些性能指标使其成为即时诊断应用的可行候选方法。
