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长微球茎菌HZ11菌株的基因组序列草图,一种具有从海藻酸盐生产生物乙醇潜力的褐藻降解细菌。

Draft genome sequence of Microbulbifer elongatus strain HZ11, a brown seaweed-degrading bacterium with potential ability to produce bioethanol from alginate.

作者信息

Sun Cong, Chen Yu-Jie, Zhang Xin-Qi, Pan Jie, Cheng Hong, Wu Min

机构信息

College of Life Sciences, Zhejiang University, Hangzhou, China.

School of Foresty and Biotechnology, Zhejiang Agriculture and Forestry University, Linan, China.

出版信息

Mar Genomics. 2014 Dec;18 Pt B:83-5. doi: 10.1016/j.margen.2014.05.009. Epub 2014 Jun 4.

Abstract

Microbulbifer elongatus strain HZ11, was a new strain of M. elongates DSM 6810(T), which has the ability to degrade brown seaweeds such as Laminaria japonica into single cell detritus particles. Here we report a high quality draft genome of M. elongatus strain HZ11, which comprises 4,223,108bp in 9 contigs with the G+C content of 56.70%. A total of 3293 protein-coding sequences were predicted, including nine alginate lyases (EC 4.2.2.3), five agarases (EC 3.2.1.81), 2-dehydro-3-deoxygluconate kinase (EC 2.7.1.45) and all enzymes involved in the Entner-Doudoroff pathway. Our results suggest that strain HZ11 has the potential ability to produce bioethanol from alginate with moderate genetic modification, which may significantly increase the yield of bioethanol from brown seaweed and the utilization rate of brown seaweeds.

摘要

长微球藻菌株HZ11是长微球藻DSM 6810(T)的一个新菌株,它能够将海带等褐藻降解为单细胞碎屑颗粒。在此,我们报告了长微球藻菌株HZ11的高质量基因组草图,其由9个重叠群组成,共4,223,108bp,G+C含量为56.70%。共预测出3293个蛋白质编码序列,包括9种海藻酸裂解酶(EC 4.2.2.3)、5种琼脂酶(EC 3.2.1.81)、2-脱氢-3-脱氧葡萄糖酸激酶(EC 2.7.1.45)以及Entner-Doudoroff途径中的所有酶。我们的结果表明,菌株HZ11经适度基因改造后具有从海藻酸盐生产生物乙醇的潜在能力,这可能会显著提高褐藻生物乙醇的产量以及褐藻的利用率。

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