Li Zhipeng, Du Zeping, Li Hebin, Chen Yanhong, Zheng Mingjing, Jiang Zedong, Du Xiping, Ni Hui, Zhu Yanbing
College of Ocean Food and Biological Engineering, Jimei University, Xiamen, 361021, China.
Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Xiamen, 361021, China.
AMB Express. 2022 Nov 5;12(1):139. doi: 10.1186/s13568-022-01482-y.
Marine bacterium Microbulbifer sp. ALW1 was revealed to be able to effectively degrade Laminaria japonica thallus fragments into fine particles. Polysaccharide substrate specificity analysis indicated that ALW1 could produce extracellular alginate lyase, laminarinase, fucoidanase and cellulase. Based on alignment of the 16 S rRNA sequence with other reference relatives, ALW1 showed the closest relationship with Microbulbifer aggregans CCB-MM1. The cell morphology and some basic physiological and biochemical parameters of ALW1 cells were characterised. ALW1 is a Gram-negative, rod- or oval-shaped, non-spore-forming and non-motile bacterium. The DNA-DNA relatedness values of ALW1 with type strains of M. gwangyangensis (JCM 17,800), M. aggregans (JCM 31,875), M. maritimus (JCM 12,187), M. okinawensis (JCM 16,147) and M. rhizosphaerae (DSM 28,920) were 28.9%, 43.3%, 41.2%, 35.4% and 45.6%, respectively. The major cell wall sugars of ALW1 were determined to be ribose and galactose, which differed from other closely related species. These characteristics indicated that ALW1 could be assigned to a separate species of the genus Microbulbifer. The complete genome of ALW1 contained one circular chromosome with 4,682,287 bp and a GC content of 56.86%. The putative encoded proteins were categorised based on their functional annotations. Phenotypic, physiological, biochemical and genomic characterisation will provide insights into the many potential industrial applications of Microbulbifer sp. ALW1.Key points.
海洋细菌微小杆菌属菌株ALW1被发现能够有效地将海带叶状体碎片降解为细颗粒。多糖底物特异性分析表明，ALW1能够产生胞外褐藻胶裂解酶、海带多糖酶、岩藻依聚糖酶和纤维素酶。基于16S rRNA序列与其他参考亲缘种的比对，ALW1与聚集微小杆菌CCB-MM1的关系最为密切。对ALW1细胞的细胞形态以及一些基本生理生化参数进行了表征。ALW1是革兰氏阴性菌，呈杆状或椭圆形，不形成芽孢，无运动性。ALW1与光阳微小杆菌（JCM 17800）、聚集微小杆菌（JCM 31875）、海生微小杆菌（JCM 12187）、冲绳微小杆菌（JCM 16147）和根际微小杆菌（DSM 28920）模式菌株的DNA-DNA相关性值分别为28.9%、43.3%、41.2%、35.4%和45.6%。确定ALW1的主要细胞壁糖类为核糖和半乳糖，这与其他密切相关的物种不同。这些特征表明，ALW1可归为微小杆菌属的一个独立物种。ALW1的完整基因组包含一条环状染色体，长度为4682287 bp，GC含量为56.86%。根据功能注释对推定编码的蛋白质进行分类。表型、生理、生化和基因组特征将为微小杆菌属菌株ALW1的许多潜在工业应用提供见解。要点。
