State Key Laboratory of Organic Geochemistry, CAS Centre for Pearl River Delta Environmental Pollution and Control Research, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China.
State Key Laboratory of Organic Geochemistry, CAS Centre for Pearl River Delta Environmental Pollution and Control Research, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China.
Sci Total Environ. 2014 Aug 15;490:708-14. doi: 10.1016/j.scitotenv.2014.05.060. Epub 2014 Jun 5.
The aim of this study was to investigate the contamination profiles of tetracycline, sulfonamide, and macrolide resistance genes, as well as integrons in sediments of Dongjiang River basin of South China by real time quantitative polymerase chain reaction. sul2 was the most abundant resistance gene, with the average concentration of 6.97×10(8) copies/g and 1.00×10(8) copies/g in the dry and wet seasons, respectively, followed by ermF, sul3, sul1, intI1, tetA, ermB, tetX, tetM, tetQ, tetO, tetW, tetS, ermC, and tetB. The abundance of intI2 gene was the lowest in the sediment samples. Significant correlations existed between the ARGs and sediment properties as well as metals (Cu and Zn) and corresponding antibiotic classes, suggesting that the contamination of ARGs is related to chemical pollution of the sediments in the river basin. Principal component analysis showed distinct groupings of the sampling sites, reflecting that human activities are the key player in the dissemination of ARGs in the catchment environment.
本研究旨在通过实时定量聚合酶链反应（qPCR）调查中国南方东江流域沉积物中四环素、磺胺类和大环内酯类耐药基因以及整合子的污染状况。sul2 是最丰富的耐药基因，其在旱季和雨季的平均浓度分别为 6.97×10(8)拷贝/g 和 1.00×10(8)拷贝/g，其次是 ermF、sul3、sul1、intI1、tetA、ermB、tetX、tetM、tetQ、tetO、tetW、tetS、ermC 和 tetB。沉积物样品中 intI2 基因的丰度最低。ARGs 与沉积物特性以及金属（Cu 和 Zn）和相应抗生素类之间存在显著相关性，表明 ARGs 的污染与流域沉积物的化学污染有关。主成分分析显示采样点明显分组，反映出人类活动是流域环境中 ARGs 传播的关键因素。
