Key Laboratory of Aquatic Botany and Watershed Ecology, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, 430074, China.
Key Laboratory of Aquatic Botany and Watershed Ecology, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, 430074, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
Chemosphere. 2017 Jul;178:19-25. doi: 10.1016/j.chemosphere.2017.03.041. Epub 2017 Mar 14.
Freshwater lakes provided an ideal media for the accumulation and propagation of antibiotic resistance genes (ARGs), because they were susceptible to anthropogenic impacts. Land reclamation and urbanization exerted severe anthropogenic impacts on lakes from middle and lower reaches of the Yangtze River, China over the past decades. In this study, 15 lakes in the region were selected to understand the level and variability of ARGs. Proportion of different land use types was applied to display the land reclamation and urbanization around each lake. For sulfonamide resistance (sul) genes, sul1 had the highest relative abundance in sediments, with maximum 2.11 × 10 copies/16SrRNA copy in Gehu Lake. For tetracycline resistance (tet) genes, tetG had the highest average value of relative abundance (4.74 × 10 copies/16SrRNA copy), followed by tetB, tetA, tetQ and tetM. Class I integron (intI1) played an important role in acquisition and dissemination of sul1 and tetG. Sediment characteristics (moisture, density, total nitrogen, total carbon, ammonium, and nitrate) were found to have no significant effect on ARG distribution. Taihu Lake and Yangcheng Lake which exhibited high sul and tet genes had the high proportion of built-up land use.
淡水湖泊为抗生素耐药基因（ARGs）的积累和传播提供了理想的媒介，因为它们容易受到人为影响。在过去几十年里，中国长江中下游地区的围湖造地和城市化对湖泊造成了严重的人为影响。在这项研究中，选择了该地区的 15 个湖泊来了解 ARGs 的水平和可变性。不同土地利用类型的比例用于显示每个湖泊周围的围湖造地和城市化情况。对于磺胺类耐药（sul）基因，sul1 在沉积物中的相对丰度最高，在 Gehu 湖中最高可达 2.11×10 copies/16SrRNA 拷贝。对于四环素耐药（tet）基因，tetG 的相对丰度平均值最高（4.74×10 copies/16SrRNA 拷贝），其次是 tetB、tetA、tetQ 和 tetM。I 类整合子（intI1）在 sul1 和 tetG 的获取和传播中起着重要作用。沉积物特性（水分、密度、总氮、总碳、铵和硝酸盐）对 ARG 分布没有显著影响。太湖和阳澄湖表现出高 sul 和 tet 基因的比例，其建设用地比例也很高。
