Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei, Taiwan.
Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei, Taiwan.
Mater Sci Eng C Mater Biol Appl. 2014 Aug 1;41:152-60. doi: 10.1016/j.msec.2014.04.048. Epub 2014 Apr 26.
Stem cells from human exfoliated deciduous teeth (SHEDs) have been considered as alternative sources of adult stem cells in tissue engineering because of their potential to differentiate into multiple cell lineages. Strontium has an important function in bone remodeling because it can simulate bone formation and decrease bone resorption. In this study, the effects of strontium phosphate on the osteogenic differentiation of SHEDs were investigated. Strontium phosphate was found to enhance the osteogenic differentiation of SHEDs with up-regulated osteoblast-related gene expression. The proliferation of SHEDs was slightly inhibited by chitosan scaffolds; however, type-I collagen expression, alkaline phosphatase activity, and calcium deposition on chitosan scaffolds containing strontium were significantly enhanced. Furthermore, cells seeded in a 3D scaffold under dynamic culture at an optimal fluid rate might enhance cellular differentiation than static culture in osteoblastic gene expression. This experiment might provide a useful cell resource and dynamic 3D culture for tissue engineering and bone repair.
人脱落乳牙干细胞（SHED）已被认为是组织工程中成人干细胞的替代来源，因为它们具有分化为多种细胞谱系的潜力。锶在骨重塑中具有重要功能，因为它可以模拟骨形成并减少骨吸收。在这项研究中，研究了磷酸锶对 SHED 成骨分化的影响。发现磷酸锶通过上调成骨相关基因表达来增强 SHED 的成骨分化。壳聚糖支架略微抑制了 SHED 的增殖；然而，在含有锶的壳聚糖支架上，I 型胶原表达、碱性磷酸酶活性和钙沉积明显增强。此外，在最佳流速下的动态培养下接种在 3D 支架中的细胞可能比静态培养在成骨基因表达方面更能增强细胞分化。该实验可能为组织工程和骨修复提供有用的细胞资源和动态 3D 培养。
