通过对肽基转移酶中心的工程改造，形成正交的核糖体-tRNA 对。
An orthogonal ribosome-tRNA pair via engineering of the peptidyl transferase center.
机构信息
1] Department of Chemistry, Graduate School of Science, University of Tokyo, Tokyo, Japan. [2].
1] Department of Chemistry, Graduate School of Science, University of Tokyo, Tokyo, Japan. [2] [3].
出版信息
Nat Chem Biol. 2014 Jul;10(7):555-7. doi: 10.1038/nchembio.1549. Epub 2014 Jun 8.
The Watson-Crick base pairs between the 3'-terminal end of tRNAs and ribosomal RNA in the peptidyl transferase center are universally conserved. Here, we report that the introduction of compensatory mutations to Escherichia coli RNAs in this site leads to an orthogonal system independent of the wild-type counterpart, as demonstrated via the production of two peptide sequences from a single mRNA. This work thus identifies a new way to reprogram the genetic code.
在肽基转移酶中心，tRNA 的 3'-末端与核糖体 RNA 之间的 Watson-Crick 碱基对是普遍保守的。在这里，我们报告说，在这个位点引入大肠杆菌 RNA 的补偿性突变会导致一个与野生型无关的正交系统，这一点通过从单个 mRNA 生产两种肽序列得到了证明。这项工作因此确定了一种重新编程遗传密码的新方法。
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