Aarabi Mahmoud, Balakier Hanna, Bashar Siamak, Moskovtsev Sergey I, Sutovsky Peter, Librach Clifford L, Oko Richard
Department of Biomedical and Molecular Sciences, School of Medicine, Queen's University, Kingston, Ontario, Canada.
CReATe Fertility Centre, Toronto, Ontario, Canada.
Fertil Steril. 2014 Aug;102(2):440-7. doi: 10.1016/j.fertnstert.2014.05.003. Epub 2014 Jun 4.
To determine the levels of postacrosomal WW binding protein (PAWP) in the spermatozoa of men that were used clinically for intracytoplasmic sperm injection (ICSI) and to correlate them with infertility treatment outcomes.
Prospective clinical and laboratory study.
University-based laboratory and infertility clinic.
PATIENT(S): Men undergoing ICSI for the treatment of couples' infertility (n=110).
INTERVENTION(S): Quantitative analysis of sperm PAWP levels by flow cytometry and developmental analysis of PAWP expression by immunoblotting, immunofluorescence, and immunohistochemistry.
MAIN OUTCOME MEASURE(S): PAWP flow-cytometric levels and immunolocalization in spermatozoa.
RESULT(S): A strong positive correlation was found between PAWP expression levels and fertilization rates after ICSI, with high levels of PAWP being associated with higher fertilization rates; the positive correlation was independent of age, DNA fragmentation index, and other sperm parameters. PAWP expression levels were correlated with embryonic development, with high levels of PAWP being associated with a lower number of arrested embryos within 3-5 days post-ICSI. PAWP expression was detected during the late stages of human spermiogenesis in elongating spermatids, confirming previous findings in various animal models.
CONCLUSION(S): Our clinical data from infertile couples demonstrate significant correlations between sperm PAWP levels and both fertilization rates and normal embryonic development after ICSI. Considering its proposed role in the initiation of oocyte activation, we suggest that PAWP could have potential applications in the diagnosis and treatment of infertility.
测定临床上用于卵胞浆内单精子注射（ICSI）的男性精子中顶体后WW结合蛋白（PAWP）的水平，并将其与不育治疗结果相关联。
前瞻性临床和实验室研究。
大学附属医院实验室和不育症诊所。
因夫妻不育接受ICSI治疗的男性（n = 110）。
通过流式细胞术对精子PAWP水平进行定量分析，并通过免疫印迹、免疫荧光和免疫组织化学对PAWP表达进行发育分析。
精子中PAWP的流式细胞术水平和免疫定位。
发现PAWP表达水平与ICSI后的受精率之间存在强正相关，PAWP水平高与较高的受精率相关；这种正相关独立于年龄、DNA碎片化指数和其他精子参数。PAWP表达水平与胚胎发育相关，PAWP水平高与ICSI后3 - 5天内发育停滞的胚胎数量较少相关。在人类精子发生后期的伸长精子细胞中检测到PAWP表达，证实了先前在各种动物模型中的发现。
我们来自不育夫妇的临床数据表明，精子PAWP水平与ICSI后的受精率和正常胚胎发育之间存在显著相关性。考虑到其在卵母细胞激活起始中所提出的作用，我们认为PAWP在不育症的诊断和治疗中可能具有潜在应用。
