Zakaria Mohd Rafein, Fujimoto Shinji, Hirata Satoshi, Hassan Mohd Ali
Biomass Refinery Research Center, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima, 739-0046, Japan,
Appl Biochem Biotechnol. 2014 Aug;173(7):1778-89. doi: 10.1007/s12010-014-0964-5. Epub 2014 Jun 8.
Oil palm biomass, namely empty fruit bunch and frond fiber, were pretreated using a planetary ball mill. Particle sizes and crystallinity index values of the oil palm biomass were significantly reduced with extended ball mill processing time. The treatment efficiency was evaluated by the generation of glucose, xylose, and total sugar conversion yields from the pretreatment process compared to the amount of sugars from raw materials. Glucose and xylose contents were determined using high-performance liquid chromatography. An increasing trend in glucose and xylose yield as well as total sugar conversion yield was observed with decreasing particle size and crystallinity index. Oil palm frond fiber exhibited the best material yields using ball milling pretreatment with generated glucose, xylose, and total sugar conversion yields of 87.0, 81.6, and 85.4%, respectively. In contrast, oil palm empty fruit bunch afforded glucose and xylose of 70.0 and 82.3%, respectively. The results obtained in this study showed that ball mill-treated oil palm biomass is a suitable pretreatment method for high conversion of glucose and xylose.
油棕生物质，即空果串和叶纤维，采用行星式球磨机进行预处理。随着球磨处理时间的延长，油棕生物质的粒径和结晶度指数值显著降低。与原材料中的糖量相比，通过预处理过程中葡萄糖、木糖和总糖转化率的生成来评估处理效率。使用高效液相色谱法测定葡萄糖和木糖含量。随着粒径和结晶度指数的降低，观察到葡萄糖和木糖产率以及总糖转化率呈上升趋势。油棕叶纤维在球磨预处理中表现出最佳的物料产率，生成的葡萄糖、木糖和总糖转化率分别为87.0%、81.6%和85.4%。相比之下，油棕空果串的葡萄糖和木糖产率分别为70.0%和82.3%。本研究获得的结果表明，球磨处理的油棕生物质是一种适合高转化率生成葡萄糖和木糖的预处理方法。
