Park Myung Soo, Fong Jonathan J, Oh Seung-Yoon, Kwon Kae Kyoung, Sohn Jae Hak, Lim Young Woon
School of Biological Sciences, Seoul National University, Seoul, 151-747, South Korea.
Antonie Van Leeuwenhoek. 2014 Aug;106(2):331-45. doi: 10.1007/s10482-014-0205-5. Epub 2014 Jun 8.
The diversity of marine-derived Penicillium from Korea was investigated using morphological and multigene phylogenetic approaches, analyzing sequences of the internal transcribed spacer region, β-tubulin gene, and RNA polymerase subunit II gene. In addition, the biological activity of all isolated strains was evaluated. We tested for the extracellular enzyme activity of alginase, endoglucanase, and β-glucosidase, and antifungal activity against two plant pathogens (Colletotrichum acutatum and Fusarium oxysporum). A total of 184 strains of 36 Penicillium species were isolated, with 27 species being identified. The most common species were Penicillium polonicum (19.6 %), P. rubens (11.4 %), P. chrysogenum (11.4 %), and P. crustosum (10.9 %). The diversity of Penicillium strains isolated from soil (foreshore soil and sand) and marine macroorganisms was higher than the diversity of strains isolated from seawater. While many of the isolated strains showed alginase and β-glucosidase activity, no endoglucanase activity was found. More than half the strains (50.5 %) showed antifungal activity against at least one of the plant pathogens tested. Compared with other strains in this study, P. citrinum (strain SFC20140101-M662) showed high antifungal activity against both plant pathogens. The results reported here expand our knowledge of marine-derived Penicillium diversity. The relatively high proportion of strains that showed antifungal and enzyme activity demonstrates that marine-derived Penicillium have great potential to be used in the production of natural bioactive products for pharmaceutical and/or industrial use.
利用形态学和多基因系统发育方法，通过分析内部转录间隔区、β-微管蛋白基因和RNA聚合酶亚基II基因的序列，对来自韩国的海洋青霉多样性进行了研究。此外，还评估了所有分离菌株的生物活性。我们测试了藻酸酶、内切葡聚糖酶和β-葡萄糖苷酶的胞外酶活性，以及对两种植物病原体（尖孢炭疽菌和尖孢镰刀菌）的抗真菌活性。共分离出36种青霉属的184株菌株，鉴定出27种。最常见的种类是波兰青霉（19.6%）、红青霉（11.4%）、产黄青霉（11.4%）和皮落青霉（10.9%）。从土壤（前滨土壤和沙子）和海洋大型生物中分离出的青霉菌株的多样性高于从海水中分离出的菌株的多样性。虽然许多分离菌株显示出藻酸酶和β-葡萄糖苷酶活性，但未发现内切葡聚糖酶活性。超过一半的菌株（50.5%）对至少一种测试的植物病原体显示出抗真菌活性。与本研究中的其他菌株相比，桔青霉（菌株SFC20140101-M662）对两种植物病原体均显示出高抗真菌活性。此处报道的结果扩展了我们对海洋青霉多样性的认识。显示出抗真菌和酶活性的菌株比例相对较高，这表明海洋青霉在生产用于制药和/或工业用途的天然生物活性产品方面具有巨大潜力。
