Hao Lijun, Xie Yuxi, Wu Guikai, Cheng Aibin, Liu Xiaogang, Zheng Rongjuan, Huo Hong, Zhang Junwei
Department of Gastroenterology, Tangshan Gongren Hospital, Tangshan 06300, China.
Department of Intensive Care Unit, Hebei United University Affiliated Hospital, Tangshan 06300, China.
Evid Based Complement Alternat Med. 2015;2015:418023. doi: 10.1155/2015/418023. Epub 2015 Apr 16.
We investigated the effects of Hericium erinaceus (HEM) on liver injury induced by acute alcohol administration in mice. Mice received ethanol (5 g/kg BW) by gavage every 12 hrs for a total of 3 doses. HEM (200 mg/kg BW) was gavage before ethanol administration. Subsequent serum alanine aminotransferase (ALT) level, aspartate aminotransaminase (AST) level, Maleic dialdehyde (MDA) level, hepatic total antioxidant status (TAOS), and activated nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) were determined by ELISA and immunohistochemistry, respectively. HEM administration markedly (P < 0.05) decreased serum ALT, AST, and MDA levels. The hepatic histopathological observations showed that HEM had a relatively significant role in mice model, which had alcoholic liver damage. In conclusion, we observed that HEM (200 mg/kg BW) supplementation could restrain the hepatic damage caused by acute alcohol exposure.
我们研究了猴头菌（HEM）对急性酒精给药诱导的小鼠肝损伤的影响。小鼠每12小时经口灌胃给予乙醇（5 g/kg体重），共3剂。在给予乙醇前经口灌胃给予HEM（200 mg/kg体重）。随后分别通过酶联免疫吸附测定（ELISA）和免疫组织化学法测定血清丙氨酸氨基转移酶（ALT）水平、天冬氨酸氨基转移酶（AST）水平、丙二醛（MDA）水平、肝脏总抗氧化状态（TAOS）以及活化B细胞核因子κB（NF-κB）。给予HEM后，血清ALT、AST和MDA水平显著降低（P < 0.05）。肝脏组织病理学观察表明，HEM在酒精性肝损伤小鼠模型中具有相对显著的作用。总之，我们观察到补充HEM（200 mg/kg体重）可以抑制急性酒精暴露引起的肝脏损伤。
