Sheu Shi-Yuan, Wang Wen-Ling, Fu Yuan-Tsung, Lin Sheng-Chuan, Lei Yi-Chih, Liao Jeng-Hao, Tang Nou-Ying, Kuo Tzong-Fu, Yao Chun-Hsu
School of Chinese Medicine, China Medical University, Taichung, Taiwan; School of Medicine, Chung Shan Medical University, Taichung, Taiwan; Department of Integrated Chinese and Western Medicine, Chung Shan Medical University Hospital, Taichung, Taiwan.
School of Chinese Medicine, China Medical University, Taichung, Taiwan; Department of Chinese Internal Medicine, China Medical University Hospital, Taichung, Taiwan.
Burns. 2014 Dec;40(8):1679-88. doi: 10.1016/j.burns.2014.04.023. Epub 2014 Jun 5.
This was a novel, prospective and interventional animal study designed to develop and evaluate a new infliction device for the experimental burn model. Four paired sets of contact burns measuring 36mm diameter were inflicted on the dorsum of an anesthetized pig using a stainless steel round bar heated up to 80-110°C. The bar was applied using a push-pull force gauge designed to control 1kgf mechanical force applied to the skin for a period of 20s. The left dorsum was used for macroscopic observation and the right dorsum was used for histopathological evaluation. A total of eight burns were covered with moist saline dressings and given daily treatments of xylocaine (lidocaine HCl) gel. This procedure was followed for a period of 24 days. Full-thickness biopsies were obtained for histologic analysis to determine the extent of injury. Statistical analysis showed a high correlation between the exposure temperature and histopathological assessment. The results found the depth of injury to the collagen (Seg1) correlated with the temperature (Ti) at which the burns was inflicted, Seg1=0.038Ti-2.57 (r=0.973, P<0.05). Also, the histological studies show a high correlation between the depth of collagen denaturation in wounds and the exposure temperature, Seg1=0.0268Ti-0.165 (r=0.991, P<0.05). This model is useful to assess more closely the therapeutic agents used for wound healing in experimental burn wounds.
这是一项新颖的、前瞻性的干预性动物研究，旨在开发和评估一种用于实验性烧伤模型的新型致伤装置。使用加热至80 - 110°C的不锈钢圆棒，在麻醉猪的背部造成四对直径为36mm的接触性烧伤。使用设计用于控制1kgf机械力并持续作用于皮肤20秒的推拉力计来施加该圆棒。猪背部左侧用于宏观观察，右侧用于组织病理学评估。总共八处烧伤均覆盖湿盐水敷料，并每日用赛罗卡因（盐酸利多卡因）凝胶进行治疗。此操作持续24天。获取全层活检组织进行组织学分析以确定损伤程度。统计分析表明暴露温度与组织病理学评估之间存在高度相关性。结果发现胶原蛋白损伤深度（Seg1）与烧伤时的温度（Ti）相关，Seg1 = 0.038Ti - 2.57（r = 0.973，P < 0.05）。此外，组织学研究表明伤口中胶原蛋白变性深度与暴露温度之间存在高度相关性，Seg1 = 0.0268Ti - 0.165（r = 0.991，P < 0.05）。该模型有助于更密切地评估用于实验性烧伤创面愈合的治疗药物。
