INRA UMR 1121 "Agronomie & Environnement" Nancy-Colmar, TSA 40602, 54518 Vandoeuvre Cedex, France; Université de Lorraine UMR 1121 "Agronomie & Environnement" Nancy-Colmar, TSA 40602, 54518 Vandoeuvre Cedex, France.
Université de Lorraine, Laboratoire d'Ingénierie des Biomolécules, TSA 40602, 54518 Vandoeuvre Cedex, France.
Plant Sci. 2014 Jul;224:62-73. doi: 10.1016/j.plantsci.2014.04.010. Epub 2014 Apr 18.
Phenolics are implicated in the defence strategies of many plant species rendering their concentration increase of putative practical interest in the field of crop protection. Little attention has been given to the nature, concentration and distribution of phenolics within vegetative organs of tomato (Solanum lycopersicum. L) as compared to fruits. In this study, we extensively characterized the phenolics in leaves, stems and roots of nine tomato cultivars using high-performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry (LC-MS(n)) and assessed the impact of low nitrogen (LN) availability on their accumulation. Thirty-one phenolics from the four sub-classes, hydroxycinnamoyl esters, flavonoids, anthocyanins and phenolamides were identified, five of which had not previously been reported in these tomato organs. A higher diversity and concentration of phenolics was found in leaves than in stems and roots. The qualitative distribution of these compounds between plant organs was similar for the nine cultivars with the exception of Micro-Tom because of its significantly higher phenolic concentrations in leaves and stems as compared to roots. With few exceptions, the influence of the LN treatment on the three organs of all cultivars was to increase the concentrations of hydroxycinnamoyl esters, flavonoids and anthocyanins and to decrease those of phenolamides. This impact of LN was greater in roots than in leaves and stems. Nitrogen nutrition thus appears as a means of modulating the concentration and composition of organ phenolics and their distribution within the whole plant.
酚类化合物与许多植物物种的防御策略有关，使它们的浓度增加在作物保护领域具有潜在的实际意义。与果实相比，人们对番茄（Solanum lycopersicum. L）营养器官中酚类化合物的性质、浓度和分布关注较少。在这项研究中，我们使用高效液相色谱-电喷雾串联质谱（LC-MS(n)）对 9 个番茄品种的叶片、茎和根中的酚类化合物进行了广泛的表征，并评估了低氮（LN）供应对其积累的影响。从四个亚类中鉴定出 31 种酚类化合物，分别为羟基肉桂酸酯、类黄酮、花色苷和酚酰胺，其中 5 种在这些番茄器官中以前没有报道过。叶片中的酚类化合物种类和浓度均高于茎和根。除 Micro-Tom 外，这 9 个品种的这些化合物在植物器官之间的定性分布相似，因为与根相比，Micro-Tom 的叶片和茎中的酚类化合物浓度明显更高。除少数例外，LN 处理对所有品种的三个器官的影响是增加羟基肉桂酸酯、类黄酮和花色苷的浓度，降低酚酰胺的浓度。这种 LN 的影响在根中大于在叶和茎中。氮营养似乎是调节器官酚类化合物浓度和组成及其在整个植物中分布的一种手段。
