Yang Yanzhu, Shi Sanyuan, Ding Qian, Chen Jian, Peng Jinliang, Xu Yuhong
School of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200240, People's Republic of China.
J Biomed Mater Res A. 2015 Mar;103(3):1045-52. doi: 10.1002/jbm.a.35255. Epub 2014 Jun 16.
Poly(D,L-lactide-co-glycolide) (PLGA) is widely used in a variety of tissue engineering and drug delivery applications due to its biodegradability and biocompatibility. But PLGA surfaces are usually hydrophobic which limited the loading and seeding capacities for cells, especially semiadherent immune cells. In this paper we described an attempt to improve the hydrophilicity and surface architecture for accommodating dendritic cells (DCs) that are widely used as professional antigen presenting cells in immune therapy of cancer and other diseases. The 3D porous PLGA scaffold was made by solvent casting/salt leaching of PLGA blended with surface functionalized multiwalled carbon nanotubes (F-MWCNTs). The incorporation and dispersion of F-MWCNT in the scaffold structures resulted in not only improved surface hydrophilicity but also nanoscale surface structure that would provide a preferable microenvironment for DCs attachment. We think such a scaffold material may be more desirable for immune cell delivery for immunotherapy.
聚（D，L-丙交酯-共-乙交酯）（PLGA）因其生物可降解性和生物相容性而被广泛应用于各种组织工程和药物递送应用中。但PLGA表面通常是疏水的，这限制了细胞的负载和接种能力，尤其是半粘附性免疫细胞。在本文中，我们描述了一种尝试，旨在改善亲水性和表面结构，以容纳树突状细胞（DCs），这些细胞在癌症和其他疾病的免疫治疗中被广泛用作专业抗原呈递细胞。三维多孔PLGA支架是通过将PLGA与表面功能化的多壁碳纳米管（F-MWCNTs）混合后进行溶剂浇铸/盐析法制备而成。F-MWCNT在支架结构中的掺入和分散不仅提高了表面亲水性，还形成了纳米级表面结构，为DCs附着提供了更适宜的微环境。我们认为这种支架材料可能更适合用于免疫治疗中的免疫细胞递送。
