Department of Biomaterials, College of Materials, Xiamen University, Key Laboratory of Biomedical Engineering of Fujian Province, Research Center of Biomedical Engineering Technology of Xiamen City, Xiamen 361005, PR China.
Colloids Surf B Biointerfaces. 2011 Apr 1;83(2):367-75. doi: 10.1016/j.colsurfb.2010.12.011. Epub 2010 Dec 15.
Biodegradable poly(lactic-co-glycolic acid) (PLGA)/carboxyl-functionalized multi-walled carbon nanotube (c-MWCNT) nanocomposites were successfully prepared via solvent casting technique. Rat bone marrow-derived mesenchymal stem cells (MSCs) were employed to assess the biocompatibility of the nanocomposites in vitro. Scanning electron microscopy (SEM) observations revealed that c-MWCNTs gave a better dispersion than unmodified MWCNTs in the PLGA matrix. Surface properties were determined by means of static contact angle, X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM) analysis. The presence of c-MWCNTs increased the mechanical properties of the nanocomposites. Seven-week period in vitro degradation test showed the addition of c-MWCNTs accelerated the hydrolytic degradation of PLGA. In addition, SEM proved that the cells could adhere to and spread on films via cytoplasmic processes. Compared with control groups, MSCs cultured onto PLGA/c-MWCNT nanocomposites exhibited better adhesion and viability and also displayed significantly higher production levels of alkaline phosphatase (ALP) over 21 days culture. These results demonstrated that c-MWCNTs modified PLGA films were beneficial for promoting cell growth and inducing MSCs to differentiate into osteoblasts. This work presented here had potential applications in the development of 3-D scaffolds for bone tissue engineering.
可生物降解的聚（乳酸-共-乙醇酸）（PLGA）/羧基功能化多壁碳纳米管（c-MWCNT）纳米复合材料通过溶剂浇铸技术成功制备。大鼠骨髓间充质干细胞（MSCs）被用于体外评估纳米复合材料的生物相容性。扫描电子显微镜（SEM）观察表明，c-MWCNTs 在 PLGA 基质中的分散性优于未修饰的 MWCNTs。通过静态接触角、X 射线光电子能谱（XPS）和原子力显微镜（AFM）分析来确定表面性能。c-MWCNTs 的存在提高了纳米复合材料的机械性能。7 周的体外降解试验表明，添加 c-MWCNTs 加速了 PLGA 的水解降解。此外，SEM 证明细胞可以通过细胞质突起附着并在薄膜上扩展。与对照组相比，在 PLGA/c-MWCNT 纳米复合材料上培养的 MSCs 表现出更好的粘附性和活力，并且在 21 天的培养过程中碱性磷酸酶（ALP）的产量也显著更高。这些结果表明，c-MWCNTs 修饰的 PLGA 薄膜有利于促进细胞生长并诱导 MSCs 向成骨细胞分化。本工作为骨组织工程 3-D 支架的开发提供了应用前景。
