Ozasa Yasuhiro, Gingery Anne, Thoreson Andrew R, An Kai-Nan, Zhao Chunfeng, Amadio Peter C
Division of Orthopedic Research and Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN.
Division of Orthopedic Research and Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN.
J Hand Surg Am. 2014 Sep;39(9):1706-13. doi: 10.1016/j.jhsa.2014.05.005. Epub 2014 Jun 6.
To investigate the ability of muscle-derived stem cells (MDSCs) supplemented with growth and differentiation factor-5 (GDF-5) to improve tendon healing compared with bone marrow stromal cells (BMSCs) in an in vitro tendon culture model.
Eighty canine flexor digitorum profundus tendons were assigned into 5 groups: repaired tendon (1) without gel patch interposition (no cell group), (2) with BMSC-seeded gel patch interposition (BMSC group), (3) with MDSC-seeded gel patch interposition (MDSC group), (4) with GDF-5-treated BMSC-seeded gel patch interposition (BMSC+GDF-5 group), and (5) with GDF-5-treated MDSC-seeded gel patch interposition (MDSC+GDF-5 group). After culturing for 2 or 4 weeks, the failure strength of the healing tendons was measured. The tendons were also evaluated histologically.
The failure strength of the repaired tendon in the MDSC+GDF-5 group was significantly higher than that of the non-cell and BMSC groups. The stiffness of the repaired tendons in the MDSC+GDF-5 group was significantly higher than that of the non-cell group. Histologically, the implanted cells became incorporated into the original tendon in all 4 cell-seeded groups.
Interposition of a multilayered GDF-5 and MDSC-seeded collagen gel patch at the repair site enhanced tendon healing compared with a similar patch using BMSC. However, this increase in vitro was relatively small. In the clinical setting, differences between MDSC and BMSC may not be substantially different, and it remains to be shown that such methods might enhance the results of an uncomplicated tendon repair clinically.
Muscle-derived stem cell implantation and administration of GDF-5 may improve the outcome of tendon repair.
在体外肌腱培养模型中，研究添加生长分化因子-5（GDF-5）的肌肉源性干细胞（MDSCs）与骨髓基质细胞（BMSCs）相比促进肌腱愈合的能力。
将80条犬指深屈肌腱分为5组：（1）无凝胶贴片植入的修复肌腱（无细胞组）；（2）有接种BMSC的凝胶贴片植入的修复肌腱（BMSC组）；（3）有接种MDSC的凝胶贴片植入的修复肌腱（MDSC组）；（4）有经GDF-5处理的接种BMSC的凝胶贴片植入的修复肌腱（BMSC+GDF-5组）；（5）有经GDF-5处理的接种MDSC的凝胶贴片植入的修复肌腱（MDSC+GDF-5组）。培养2周或4周后，测量愈合肌腱的断裂强度。对肌腱进行组织学评估。
MDSC+GDF-5组修复肌腱的断裂强度显著高于无细胞组和BMSC组。MDSC+GDF-5组修复肌腱的刚度显著高于无细胞组。组织学上，在所有4个接种细胞组中，植入细胞均融入原始肌腱。
与使用BMSC的类似贴片相比，在修复部位植入多层GDF-5和接种MDSC的胶原凝胶贴片可增强肌腱愈合。然而，这种体外增加相对较小。在临床环境中，MDSC和BMSC之间的差异可能不大，且仍有待证明此类方法在临床上可能会改善单纯肌腱修复的结果。
肌肉源性干细胞植入和GDF-5给药可能改善肌腱修复的结果。
