Biomechanics Laboratory, Division of Orthopedic Research, Mayo Clinic, Rochester, MN 55905, USA.
Med Eng Phys. 2009 Dec;31(10):1271-5. doi: 10.1016/j.medengphy.2009.08.004. Epub 2009 Sep 6.
The purpose of this study was to investigate the effect of bone marrow stromal cells (BMSCs) on tendon healing in a canine ex vivo model. Bone marrow was harvested and BMSCs were isolated and cultured according to established protocols. Cells were seeded into 0.5mg/ml collagen gels and cultured for 24h to allow gel contraction, and then implanted between the lacerated ends of repaired flexor digitorum profundus tendons. Tendons repaired with a gel patch alone and without a gel patch served as control groups. After 2 and 4 weeks in culture, the repaired tendons were evaluated for breaking strength and stiffness. Cell viability was assessed by labeling the cells with PKH26 red fluorescent cell linker. The maximal strength and stiffness of repaired tendons with the BMSC-seeded patch were significantly higher than the repaired tendons without a patch or with a patch without cells, at both 2 and 4 weeks (p<0.05). Viable BMSC were present between the cut tendon ends at both 2 and 4 weeks. We conclude that BMSC-seeded gel patch transplantation has the potential to enhance flexor tendon healing, and we plan to investigate this effect in vivo.
本研究旨在探讨骨髓基质细胞（BMSCs）对犬体外模型肌腱愈合的影响。按照既定方案采集骨髓，分离并培养 BMSCs。将细胞接种到 0.5mg/ml 的胶原凝胶中，培养 24 小时以允许凝胶收缩，然后将其植入修复的指深屈肌腱的撕裂端之间。单独使用凝胶补片和不使用凝胶补片修复的肌腱作为对照组。在培养 2 和 4 周后，评估修复后的肌腱的断裂强度和刚度。通过用 PKH26 红色荧光细胞连接剂标记细胞来评估细胞活力。在第 2 和第 4 周时，与没有补片或没有细胞的补片修复的肌腱相比，接种 BMSC 的补片修复的肌腱的最大强度和刚度显著更高（p<0.05）。在第 2 和第 4 周时，活的 BMSC 存在于切断的肌腱末端之间。我们得出结论，BMSC 接种凝胶补片移植具有增强屈肌腱愈合的潜力，我们计划在体内研究这种效果。
