Bajpai Anamika, Prasad Kashi Nath, Mishra Priyanka, Singh Aloukick Kumar, Gupta Rakesh Kumar, Ojha Bal Krishan
Department of Microbiology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, India.
Department of Microbiology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, India.
J Neuroimmunol. 2014 Aug 15;273(1-2):96-102. doi: 10.1016/j.jneuroim.2014.05.009. Epub 2014 May 24.
Brain abscess is a focal suppurative process. Host inflammatory response in Gram type and specific bacteria has not been studied in brain abscess. A total of 57 brain abscess patients with monomicrobial infections were studied for Th1 (TNF-α, IFN-γ, IL1-β), Th2 (IL-4, IL-10) and Th17 (IL-17, IL-23) cytokine response by reverse-transcriptase PCR and ELISA. Th1 and Th17 cytokines were significantly elevated in Gram positive (Staphylococcus aureus and Streptococcus intermedius) and Th2 cytokine (IL-10) in Gram negative (Bacteroides fragilis and Escherichia coli) infections (p<0.05). Cytokine levels were significantly higher in abscess than blood (p<0.001). Elevated levels of several inflammatory cytokines (TNF-α, IFN-γ, IL1-β, IL-17 and IL-23) were associated with the duration of symptoms; predisposing factors also influenced the levels of several cytokines. The expression of inflammatory cytokines in abscess was influenced by the bacterial pathogen, duration of symptoms and predisposing factors. Local milieu of brain plays significant role in secretion of various cytokines.
脑脓肿是一种局限性化脓性病变。脑脓肿中宿主对革兰氏类型及特定细菌的炎症反应尚未得到研究。通过逆转录聚合酶链反应和酶联免疫吸附测定，对57例单微生物感染的脑脓肿患者进行了Th1（肿瘤坏死因子-α、干扰素-γ、白细胞介素-1β）、Th2（白细胞介素-4、白细胞介素-10）和Th17（白细胞介素-17、白细胞介素-23）细胞因子反应的研究。在革兰氏阳性菌（金黄色葡萄球菌和中间型链球菌）感染中，Th1和Th17细胞因子显著升高，而在革兰氏阴性菌（脆弱拟杆菌和大肠杆菌）感染中，Th2细胞因子（白细胞介素-10）升高（p<0.05）。脓肿中的细胞因子水平显著高于血液中的水平（p<0.001）。几种炎症细胞因子（肿瘤坏死因子-α、干扰素-γ、白细胞介素-1β、白细胞介素-17和白细胞介素-23）水平的升高与症状持续时间相关；易感因素也会影响几种细胞因子的水平。脓肿中炎症细胞因子的表达受细菌病原体、症状持续时间和易感因素的影响。脑局部微环境在各种细胞因子的分泌中起重要作用。
