International Tomography Center SB RAS, Institutskaya 3a, Novosibirsk 630090, Russia; Novosibirsk State University, Pirogova 2, Novosibirsk 630090, Russia.
International Tomography Center SB RAS, Institutskaya 3a, Novosibirsk 630090, Russia.
Exp Eye Res. 2014 Aug;125:71-8. doi: 10.1016/j.exer.2014.05.016. Epub 2014 Jun 5.
This work is the first comprehensive report on the quantitative metabolomic composition of the rat lens. Quantitative metabolomic profiles of lenses were acquired with the combined use of high-frequency nuclear magnetic resonance (NMR) and high-performance liquid chromatography with high-resolution mass-spectrometric detection (LC-MS) methods. More than forty low molecular weight compounds found in the lens have been reliably identified and quantified. The most abundant metabolites in the 3-month-old Wistar rat lens are taurine, hypotaurine, lactate, phosphocholine and reduced glutathione. The analysis of age-related changes in the lens metabolomic composition shows a gradual decrease of the content of most metabolites. This decrease is the most pronounced between 1 and 3 months, which probably corresponds to the completion of the lens maturation in one-month-old rats and to the high rate of the young lens growth. The enhanced levels of tryptophan, tyrosine, carnitine, glycerophosphate, GSH and GSSG were found in lenses of senescence-accelerated OXYS rats; for some metabolites, this effect may probably be attributed to the compensatory response to oxidative stress.
这是第一篇关于大鼠晶状体定量代谢组学组成的综合报告。采用高频核磁共振（NMR）和高效液相色谱-高分辨率质谱检测（LC-MS）联用的方法获得了晶状体的定量代谢组学图谱。已可靠地鉴定和定量了晶状体中发现的四十多种低分子量化合物。在 3 个月大的 Wistar 大鼠晶状体中，含量最丰富的代谢物是牛磺酸、次牛磺酸、乳酸盐、磷酸胆碱和还原型谷胱甘肽。分析晶状体代谢组组成的年龄相关性变化表明，大多数代谢物的含量逐渐下降。这种下降在 1 至 3 个月之间最为明显，这可能与 1 个月大的大鼠晶状体成熟完成以及年轻晶状体生长速度快有关。在快速老化 OXYS 大鼠的晶状体中发现色氨酸、酪氨酸、肉碱、甘油磷酸、GSH 和 GSSG 的水平升高；对于一些代谢物，这种影响可能归因于对氧化应激的补偿反应。
