Tavassoli-Hojjati Sara, Aliasghar Elham, Babaki Fatemeh Ahmadian, Emadi Fatemeh, Parsa Maliheh, Tavajohi Shohreh, Ahmadyar Maryam, Ostad Seyed Nasser
Assistant Professor, Department of pediatric Dentistry, Dental school, Shahed University, Tehran, Iran.
Dentist, Department of pediatric Dentistry, Dental school, Shahed University, Tehran, Iran.
J Dent (Tehran). 2014 Mar;11(2):225-32. Epub 2014 Mar 31.
There is evidence indicating that pomegranate juice contains many of the essential properties necessary to retain cell viability and cell proliferation. These properties indicate that pomegranate juice is a suitable storage medium for avulsed teeth. However, this idea has not yet been tested. In this study, the capacity of pomegranate juice (PJ) as a storage medium for retaining avulsed teeth was evaluated.
PDL fibroblasts were obtained from healthy human premolars and cultured in Dulbecco's Modified Eagle's Medium (DMEM). Cultured cells were subjected to different concentrations of pomegranate juice (PJ), 1% Hank's balanced salt solution (HBSS) and tap water for 1, 3, 6 and 24 hours. PDL cell viability was assessed by the neutral red uptake assay.
The results indicated that 7.5% PJ was the most effective solution for maintaining PDL cell viability amongst all the experimental solution's and time intervals (P<0.05). The results also showed that 1% PJ was as effective as HBSS for maintaining PDL cell viability. The amount of cell viability increased with increasing concentration of PJ at all time intervals (P<0.001). This effect is suggestive of the proliferative potential of PJ solution.
In conclusion, PJ can be recommended as a suitable transport medium for avulsed teeth.
有证据表明石榴汁含有维持细胞活力和细胞增殖所需的许多基本特性。这些特性表明石榴汁是一种适合用于储存脱位牙的介质。然而，这一观点尚未得到验证。在本研究中，评估了石榴汁（PJ）作为脱位牙储存介质的能力。
从健康人前磨牙获取牙周膜成纤维细胞，并在杜尔贝科改良伊格尔培养基（DMEM）中培养。将培养的细胞分别置于不同浓度的石榴汁（PJ）、1%汉克平衡盐溶液（HBSS）和自来水中1、3、6和24小时。通过中性红摄取试验评估牙周膜细胞活力。
结果表明，在所有实验溶液和时间间隔中，7.5%的PJ是维持牙周膜细胞活力最有效的溶液（P<0.05）。结果还表明，1%的PJ在维持牙周膜细胞活力方面与HBSS效果相当。在所有时间间隔内，细胞活力随着PJ浓度的增加而增加（P<0.001）。这种效应提示了PJ溶液的增殖潜力。
总之，PJ可被推荐为脱位牙合适的运输介质。
