Zhu Haiyan, Shi Xunlong, Ju Dianwen, Huang Hai, Wei Wei, Dong Xiaoying
Department of Biosynthesis, School of Pharmacy, Fudan University, 826 Zhangheng Road, 201203, Shanghai, China.
Inflammation. 2014 Dec;37(6):2091-8. doi: 10.1007/s10753-014-9943-9.
The purpose of this study is to investigate the anti-inflammatory effect of thalidomide (Thd) on H1N1-induced acute lung injury in mice. BALB/C mice were infected intranasally with influenza A virus (H1N1) and then treated with Thd at a dose of 100 or 200 mg/kg/day for 7 days. Weight loss and survival of mice were monitored for 14 days after virus challenge, and the serum and lung tissues were collected at 4 days for histological and biochemical analysis. The results showed that Thd significantly improved the survival rate, reduced the infiltration of inflammatory cells and cytokine (e.g., IL-6, TNF-α) and chemokine (e.g., RANTES, IP-10) levels, and inhibited activated p-NFκB p65 in infected mice. These findings suggested that Thd may attenuate H1N1-induced pulmonary injury and thus may find use in the treatment of viral diseases.
本研究的目的是探讨沙利度胺（Thd）对甲型流感病毒（H1N1）诱导的小鼠急性肺损伤的抗炎作用。将BALB/C小鼠经鼻内感染甲型流感病毒（H1N1），然后以100或200mg/kg/天的剂量用Thd治疗7天。在病毒攻击后14天监测小鼠的体重减轻和存活情况，并在第4天收集血清和肺组织进行组织学和生化分析。结果表明，Thd显著提高了存活率，减少了炎症细胞浸润以及细胞因子（如IL-6、TNF-α）和趋化因子（如RANTES、IP-10）水平，并抑制了感染小鼠中活化的p-NFκB p65。这些发现表明，Thd可能减轻H1N1诱导的肺损伤，因此可能用于治疗病毒性疾病。
