State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, PR China.
Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, PR China.
Int J Syst Evol Microbiol. 2014 Sep;64(Pt 9):2939-2943. doi: 10.1099/ijs.0.061671-0. Epub 2014 Jun 9.
A Gram-reaction-negative, non-spore-forming strain, designated 5DNS001(T), was isolated from soil of an ancient salt-extracting facility in China. Analysis of the almost-complete 16S rRNA gene sequence of the bacterium suggested that it belongs to the genus Sinomicrobium in the family Flavobacteriaceae. It exhibited highest 16S rRNA gene sequence similarity with Sinomicrobium oceani SCSIO 03483(T) (96.3 %), but less than 93 % sequence similarity with members of the genera Imtechella, Zhouia and Joostella and other recognized members of the family Flavobacteriaceae. The strain was able to hydrolyse pectin and starch by producing pectinase and α-amylase. The DNA G+C content of the strain was 42.6 mol%. The major respiratory quinone was MK-6. The major polar lipid detected in the strain was phosphatidylethanolamine. The dominant cellular fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 3 (C16 : 1ω6c/C16 : 1ω7c). Based on phenotypic, genotypic, chemotaxonomic and phylogenetic analyses, a novel species, Sinomicrobium pectinilyticum, is proposed. The type strain is 5DNS001(T) ( = CGMCC1.11000(T) = KCTC23776(T)).
一株革兰氏反应阴性、不产芽孢的菌株，命名为 5DNS001(T)，从中国一个古老的盐提取设施的土壤中分离得到。该细菌的近全长 16S rRNA 基因序列分析表明，它属于黄杆菌科 Sinomicrobium 属。它与海洋 Sinomicrobium oceani SCSIO 03483(T)（96.3%）的 16S rRNA 基因序列最相似，但与 Imtechella、Zhouia 和 Joostella 属的成员以及其他公认的黄杆菌科成员的相似度低于 93%。该菌株能够通过产生果胶酶和α-淀粉酶水解果胶和淀粉。该菌株的 DNA G+C 含量为 42.6 mol%。主要的呼吸醌是 MK-6。在该菌株中检测到的主要极性脂是磷脂酰乙醇胺。主要的细胞脂肪酸是 iso-C15：0、iso-C17：0 3-OH 和总和特征 3（C16：1ω6c/C16：1ω7c）。基于表型、基因型、化学分类和系统发育分析，提出了一个新的物种，即果胶分解 Sinomicrobium pectinilyticum。模式菌株是 5DNS001(T)（ = CGMCC 1.11000(T) = KCTC 23776(T)）。
