Liu Xiupian, Lai Qiliang, Du Yaping, Zhang Xiaorong, Zhong Huanzi, Shao Zongze
Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, State Key Laboratory Breeding Base of Marine Genetic Resources, Fujian Key Laboratory of Marine Genetic Resources, Xiamen 361005, PR China.
Int J Syst Evol Microbiol. 2019 Apr;69(4):1070-1074. doi: 10.1099/ijsem.0.003273. Epub 2019 Feb 12.
A bacterial strain, designated N-1-3-6, was isolated from a soil sample collected from the arctic regions. The cells were short rods, Gram-stain-negative, catalase- and oxidase-positive. Growth was observed with 0-12 % (w/v) NaCl, with optimal growth at 0.5-2 %, and at pH 6.0-9.0, with optimum of pH 7.0, and a growth temperature of 10-45 °C, with an optimum of 28-37 °C. Phylogenetic analysis based on the 16S rRNA gene placed N-1-3-6 in the genus Sinomicrobium with the closest relative being Sinomicrobium pectinilyticum 5DNS001, exhibiting 95.3 % 16S rRNA pairwise similarity. A polyphasic taxonomic study, including phenotypic, chemotaxonomic and molecular analyses, was performed to clarify its taxonomic position. N-1-3-6 contained MK-6 as the predominant menaquinone. Polar lipids consisted of phosphatidylethanolamine and several unidentified aminolipids, phospholipids and lipids. The principal fatty acids (>10 %) were iso-C15 : 0 (26.9 %), summed feature 3 [C16 : ω7c/ω6c (17.2 %)] and iso-C17 : 0 3-OH (14.7 %). The DNA G+C content of N-1-3-6 was 47.7 mol%. On the basis of its phenotypic and genotypic properties, strain N-1-3-6 should be classified as representing a novel species of the genus Sinomicrobium, for which the name Sinomicrobium soli sp. nov. is proposed, with the type strain N-1-3-6 (=MCCC 1A06047=KCTC 52339).
从北极地区采集的土壤样本中分离出一株细菌，命名为N-1-3-6。细胞为短杆状，革兰氏染色阴性，过氧化氢酶和氧化酶阳性。在含0-12%（w/v）NaCl的培养基中可生长，最适生长NaCl浓度为0.5-2%，pH值为6.0-9.0，最适pH值为7.0，生长温度为10-45℃，最适温度为28-37℃。基于16S rRNA基因的系统发育分析将N-1-3-6归入中华微杆菌属，与果胶分解中华微杆菌5DNS001亲缘关系最近，16S rRNA序列相似性为95.3%。进行了多相分类研究，包括表型、化学分类和分子分析，以明确其分类地位。N-1-3-6以MK-6作为主要甲基萘醌。极性脂类由磷脂酰乙醇胺和几种未鉴定的氨基脂类、磷脂和脂类组成。主要脂肪酸（>10%）为异-C15:0（26.9%）、特征性总和3 [C16:ω7c/ω6c（17.2%）]和异-C17:0 3-OH（14.7%）。N-1-3-6的DNA G+C含量为47.7 mol%。基于其表型和基因型特征，菌株N-1-3-6应被分类为中华微杆菌属的一个新物种，为此提出新名称中华土壤微杆菌（Sinomicrobium soli sp. nov.），模式菌株为N-1-3-6（=MCCC 1A06047=KCTC 52339）。
